Removal of unbound sodium dodecyl sulfate (SDS) from proteins in solution by electrophoresis through triton X-100-agarose |
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| Authors: | L.T. Lee J.E. Deas C. Howe |
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| Affiliation: | Departments of Microbiology and Tropical Medicine, Louisiana State University Medical Center, New Orleans, LA, U.S.A. |
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| Abstract: | Residual sodium dodecyl sulfate (SDS) introduces artifacts into immuno- and counter- immunoelectrophoretic analysis of proteins which have been eluted from preparative SDS-polyacrylamide gels. Unbound SDS can be removed by electrophoretic passage of eluted solutions through a barrier of Triton X-100 in agarose in which the anionic and non-ionic detergents interact to form micelles. |
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