幽门螺杆菌内毒素对小鼠成纤维细胞和人胃粘膜组织细胞的侵入和损害

应用免疫胶金技术证实幽门螺杆菌内毒素（ＨＰ—ＬＰＳ）作用于小鼠成纤维细胞后５ｍｉｎ即能侵入胞浆，６０ｍｉｎ后可在细胞核中检出ＨＰ—ＬＰＳ结合的胶金颗粒。免疫组织化学法检测结果表明，６５．４％的胃粘膜活检标本组织中有ＨＰ—ＬＰＳ存在。受ＨＰ—ＬＰＳ作用的小鼠成纤维细胞和ＨＰ—ＬＰＳ检测阳性的胃炎患者活检标本细胞均出现相似的超微结构病变，如粗面内质网扩张、膜旁核糖体颗粒脱落、线粒体嵴或外膜消失等。实验结果表明幽门螺杆菌内毒素是该菌致慢性胃炎的主要致病物质之一。

Invasion and Damage to Mouse Fibroblasts and Cells of Human Gastric Mucosa by the Endotoxin of Helicobacter Pylori

The Endotoxin of Helicobacter pylori(HP-LPS) could invase the cytoplasm in 5 minutes and the nucleus in 60 minutes of mouse fibromasts after HP-LPShad act on the cells by immuno-aurosol technique, The aurosol particles connected with HP-LPS could exist in cytomembrane, cytoplasm, nuclear membrane and gastritis. HP-LPS in cells of 65.4%of the biopsy specimen could be found by immunohistochemical method. The fibroblasts treated with HP-LPS and the cells in which HP-LPS examination was positive showed similar ultrastructure changes such as rough endoplasmic reticulum dilatation, paramenbranous ribosome particle exfoliation, motochodrial crista and outer membrane disappearance, the results showed that HPLPS was one of the major pathogenic substances of HP of chronic gastritis.