组织工程血管基质的制备及保存

摘要背景：为构建全生物化组织工程血管，制备及保存脱细胞血管基质。目的：制备组织工程血管基质，观察其保存于液氮中的可行性。方法：采用胰蛋白酶、低渗溶液、化学除垢剂法处理兔胸主动脉来制备组织工程血管基质，标本作大体、光镜及扫描电镜、透射电镜观察，并将制备的血管基质放入液氮中保存，3个月后取出作扫描电镜观察。结果与结论：经该法处理的兔血管细胞全部脱除，胶原纤维、弹性纤维无断裂，细胞外基质保持完好，液氮保存3个月后扫描电镜观察与新鲜基质无明显差别。提示酶、低渗溶液、化学除垢剂联合法是制备组织工程血管基质的较好方法，制备的血管基质可放入液氮中进行短期保存。

Preparation and preservation of tissue engineered vascular matrix

AbstractBACKGROUND: Acellular vascular matrix is prepared and preserved in order to construct complete biological tissue engineered vessels.OBJECTIVE: To prepare tissue engineered vascular matrix and to investigate the feasibility of preserving it in liquid nitrogen.METHODS: Trypsin, hypertonic solutions and chemical detergent were applied for a multi-step process to prepare acellular tissue engineered vascular matrix from rabbit thoracic aorta. The specimen was observed with macroscopic observation, light microscope, scanning electron microscope and transmission electron microscope. Then the specimen was observed by scanning electron microscope after preserved in liquid nitrogen for 3 months.RESULTS AND CONCLUSION: The cells were removed completely after this processing in rabbit blood vessels, the elastic fibers and collagen fibers were preserved well, extracellular matrix maintained intact. There were no obvious differences in scanning electron microscopy observation between the specimen preserved in liquid nitrogen for 3 months and the fresh matrix. A combined method of trypsin, hypertonic solution and chemical detergent can be a better method to prepare tissue engineered vascular matrix, and the vascular matrix could be preserved in liquid nitrogen for a short time.