| 全反式维甲酸对多种胰腺癌细胞的诱导凋亡作用 |
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| 引用本文： | 朱颖[1] 夏璐[1] 章永平[1] 张学军[2] 袁耀宗[1]. 全反式维甲酸对多种胰腺癌细胞的诱导凋亡作用[J]. 胰腺病学, 2007, 7(1): 24-27 |
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| 作者姓名： | 朱颖[1] 夏璐[1] 章永平[1] 张学军[2] 袁耀宗[1] |
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| 作者单位： | 上海交通大学医学院附属瑞金医院消化内科，上海交通大学医学院附属瑞金医院消化内科，上海交通大学医学院附属瑞金医院消化内科，中科院上海生命科学院生物化学与细胞生物学研究所分子生物实验室，上海交通大学医学院附属瑞金医院消化内科 |
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| 摘 要： | 目的观察全反式维甲酸(all-trans retinoic acid,ATRA)能否诱导人胰腺癌细胞的凋亡。方法分别将人胰腺癌细胞SW1990、PaTu8988、BxPC3和ATRA共同孵育后,用MTT法检测细胞活性,分别用流式细胞仪检测、TUNEL和透射电镜观察细胞的凋亡。结果MTT法显示,ATRA对3株人胰腺癌细胞株SW1990、PaTu8988和BxPC3的生长均有明显的抑制作用(P<0.05)。流式细胞仪的检测、TUNEL和电镜的观察结果均证实ATRA诱导后,3株胰腺癌细胞株凋亡率均高于对照(P<0.01),并随诱导时间延长而增加。结论ATRA在体外能显著抑制多株胰腺癌细胞株的生长,并促进其凋亡。
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| 关 键 词： | 胰腺肿瘤 细胞凋亡 全反式维甲酸 |
| 文章编号： | 23798064 |
| 修稿时间： | 2006-07-18 |
The effect of all-trans retinoic acid on inducing apoptosis of multiple pancreatic cancer cell lines. |
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| ZHU Ying, XIA Lu, ZHANG Yong-ping, ZHANG Xue-jun, YUAN Yao-zong.. The effect of all-trans retinoic acid on inducing apoptosis of multiple pancreatic cancer cell lines.[J]. Chinese JOurnal of Pancreatology, 2007, 7(1): 24-27 |
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| Authors: | ZHU Ying XIA Lu ZHANG Yong-ping ZHANG Xue-jun YUAN Yao-zong. |
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| Affiliation: | Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China |
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| Abstract: | Objective To study the effect of all-trans retinoic acid (ATRA) on inducing apoptosis of several pancreatic cancer cell lines in vitro. Methods Viability of pancreatic cancer cells (SW1990, Patu8988 and Bx-PC3) with or without ATRA was measured by MTT method. Apoptosis was detected by flow cytometry (FCM), TUNEL method and transmission electron microscopy (TEM). Results MTT method showed significant inhibition on cell viability after ATRA treatment (P < 0.05). Apoptotic rates were higher than control (P < 0.01) after ATRA treatment and increased in a time phase manner as observed by FCM, TUNEL and TEM. Conclusions ATRA inhibited the viability of several pancreatic cancer cell lines in vitro and induced cell apoptosis. |
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| Keywords: | Pancreatic cancer Apoptosis All-trans retinoic acid |
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