Systemic exposure of quercetin after administration of feng-liao-chang-wei-kang granules to rats |
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Authors: | Zhang Junqing Liu Xia Fu Naiguang Liu Mingsheng Tan Yinfeng |
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Affiliation: | Department of Pharmacy, Hainan Medical College, Hainan Provincial Key Laboratory of R&D of Tropical Herbs, Xueyuan Road No. 3, Haikou 571101, China |
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Abstract: | Aim of studyThe objective of this study was to observe the systemic exposure of quercetin (QCT) including its free and total concentration in rat blood samples following a single p.o. dose of Feng-Liao-Chang-Wei-Kang granules (FLCWKG).Materials and methodsSix male rats were given the FLCHKG and the serial blood samples were collected. Fully or partially validated LC/MS/MS methods were developed to analyze the resulting biosamples. Various pharmacokinetic (PK) parameters were estimated from the plasma concentration versus time data using non-compartmental methods.ResultsBoth methods for analysis of the free and total QCT plasma concentration are sensitive, specific, accurate and reliable. The PK parameters of free QCT after administration of FLCWKG, in comparison with total concentration, show a lower Cmax (32.4 ± 8.2 ng/ml versus 164 ± 49 ng/ml), a lower AUC(0 → ∞) (39.3 ± 6.9 ng/ml h versus 313 ± 16 ng/ml h), a shorter Tmax (0.17 ± 0.00 h versus 0.83 ± 1.01 h) and a similar t1/2 (3.90 ± 0.88 h versus 3.10 ± 1.94 h), MRT(0 → t) (2.27 ± 0.10 h versus 2.86 ± 0.41 h).ConclusionsFree QCT was quickly absorbed from gastrointestinal tract and circulated in blood at quite low concentration. The circulating flavonoids originating from this formula were dominantly conjugated derivatives. |
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Keywords: | FLCWKG Feng-Liao-Chang-Wei-Kang granules IS internal standard LLOQ lower limit of quantification TCM traditional Chinese medicine LC liquid chromatography t1/2 elimination half-life MRT mean duration time Cmax peak concentration Tmax the time taken to achieve peak concentration |
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