Screening of Caesalpinia bonduc leaves for antipsoriatic activity |
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Authors: | Muruganantham N Basavaraj K H Dhanabal S P Praveen T K Shamasundar N M Rao K S |
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Institution: | a Department of Phytopharmacy and Phytomedicine (TIFAC CORE HD), JSS College of Pharmacy, Rocklands, Ooty, India b Department of Dermatology and Venerology, JSS Medical College, Mysore, India c Department of Anatomy, JSS Medical College, Mysore, India d Department of Biochemistry and Nutrition, Central Food Technology and Research Institute, Mysore, India |
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Abstract: | Ethnopharmacological relevanceLeaves of Caesalpinia bonduc (L.) Roxb. (Caesalpiniaceae) have been used by traditional Siddha healer of Malabar region for psoriasis treatment.Aim of the studyTo evaluate the Caesalpinia bonduc decoction (CBD), Caesalpinia bonduc hydroalcoholic extract (CBHA) for antipsoriatic activity.Materials and methodsMouse tail test for psoriasis was used for the evaluation of antipsoriatic activity. Extracts were tested at a dose of 500 mg/kg b.w. and fractions at 250 mg/kg b.w. in Swiss albino mice. Parameters studied in the mouse tail test were changes in epidermal thickness and percentage orthokeratotic values. In vitro antiproliferant assay on HaCaT cell lines and in vitro lipoxygenase inhibition were also carried out.ResultsButanol fraction of Caesalpinia bonduc hydroalcoholic extract (CBHAB) and water fraction of Caesalpinia bonduc hydroalcoholic extract (CBHAW) produced significant orthokeratosis (p < 0.001). In relative epidermal thickness, a significant (p < 0.05) reduction with respect to control was observed in groups treated with retinoic acid, CBD, butanol fraction of Caesalpinia bonduc decoction (CBDB), water fraction of Caesalpinia bonduc hydroalcoholic extract (CBHAW). Maximum antiproliferant activity was shown by CBHA (IC50, 77.5 ± 12.7 μg/ml). In lipoxygenase inhibition assay, water fraction of Caesalpinia bonduc decoction (CBDW) showed maximum activity with an IC50 value of 164.71 ± 4.57 μg/ml.ConclusionsAmong all the tested samples only CBHAW showed good activity in the mouse tail test, antiproliferant activity in HaCaT cells and lipoxygenase inhibition assay. Other extracts and fractions showed varying degrees of activity. The present study supports the traditional use of Caesalpinia bonduc leaves for psoriasis treatment. |
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Keywords: | Caesalpinia bonduc Caesalpiniaceae Psoriasis Mouse tail test Orthokeratosis Lipoxygenase inhibition assay |
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