内皮素抗体在大鼠肾缺血/再灌注损伤中的作用

目的和方法：为探讨内皮素在肾缺血／再灌注损伤中的发病学意义，本实验在大鼠肾缺血／再灌注损伤模型上，观察了内皮素抗体对肾缺血／再灌注损伤的作用。结果：肾缺血／再灌注损伤大鼠，血清尿素氮（ＳＵＮ）、肌酐（ＳＣｒ）含量及血浆内皮素（ＰＥＴ）水平显著升高；脂质过氧化物丙二醛（ＭＤＡ）产生增多；肾小管上皮细胞明显损伤。应用内皮素单克隆抗体能显著降低ＰＥＴ水平和ＭＤＡ含量，减轻肾小管上皮细胞损伤，改善肾功能。结论：内皮素是参与肾缺血／再灌注损伤发病的重要因素之一，阻断ＥＴ的生物学作用是防治肾缺血／再灌注损伤的有效途径。

The role of ET-Ab in renal ischemia-reperfusion injury in rats

AIM and METHOD In order to evaluate the possible role of ET-Ab in the pathogenesis of renal ischemia-reperfusion injury, the effect of ET-Ab on renal ischemia-reperfusion injury was investigated on a model produced by bilateral clamping the renal artery and vein followed by reperfusion in rats.RESULTS:Contents of SUN, SCr, MDA and level of plasma endothelin (P ET ) were significantly increased in renal ischemia-reperfusion injured rats, and the impairment of renal tubular epithelial cells was observed. ET-Ab might siginificantly decreased contents of SUN, SCr, MDA and level of P ET , and attenuate the renal ischemia-reperfusion injury of rats.CONCLUSION:ET plays an important role in the pathogenesis of ischemia-reperfusion injury. Blocking the biological actions of ET might be an effective means of preventing and treating ischemia-reperfusion injury.