A new model of experimental parotitis in rats and its implication for trigeminal nociception |
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| Authors: | A.?Ogawa,K.?Ren,Y.?Tsuboi,T.?Morimoto,T.?Sato,K.?Iwata author-information" > author-information__contact u-icon-before" > mailto:iwata-k@dent.nihon-u.ac.jp" title=" iwata-k@dent.nihon-u.ac.jp" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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| Affiliation: | (1) Department of Physiology, Nihon University, School of Dentistry, 1-8-13, Kanda-surugadai, Chiyoda-ku, 101-8310 Tokyo, Japan;(2) Departments of Dental Anesthesiology, Osaka University, Graduate School, Faculty of Dentistry 1-8, Yamadaoka, 565-0871 Suita Osaka, Japan;(3) Department of Oral and Craniofacial Biological Sciences, University of Maryland Dental School, Baltimore, Maryland 21201, USA;(4) Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, 101-8310 Tokyo, Japan;(5) Institute for Oral Sciences, Division of Oral & Maxillofacial Biology, Matsumoto Dental University, Shiojiri, 399-0781 Nagano, Japan;(6) Japan Core Research for Evolutional Science and Technology (CREST), 332-0012 Kawaguchi , Japan |
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| Abstract: | A rat model of chronic parotitis was developed following a direct injection of Complete Freund's adjuvant (CFA) into the unilateral parotid gland via the parotid duct without skin incision. The nocifensive behavior, plasma extravasation in the parotid gland, and trigeminal Fos protein expression, a marker of neuronal activation, were analyzed in this model and compared to that of the saline-injected rats. A significant reduction of the escape threshold to mechanical stimulation of the lateral face on the ipsilateral side to the CFA injection was observed at 1–6 days after CFA injection as compared to that of the pre-CFA control (P<0.01). The lateral face region contralateral to the CFA injection also showed mechanical hyperalgesia at 1–6 days after injection (P<0.05). The plasma extravasation was significantly increased in the parotid gland ipsilateral to CFA injection as compared to that of the parotid gland with saline injection at 3 days after injection as shown by Evans' blue dye extravasation (P<0.05). Bilateral expression of Fos protein-like immunoreactive cells was observed in the transition zone between the trigeminal spinal nucleus interpolaris (Vi) and caudalis (Vc) and paratrigeminal nucleus (Pa5). On the other hand, a significant unilateral expression of Fos protein-positive cells was observed on the ipsilateral side of the upper cervical (C2) dorsal horn (P<0.05). This model of parotitis can be used to study trigeminal pain mechanisms associated with sialadenitis. A unique feature of this preparation is that the inflammation was limited to the parotid gland after intraductal injection of CFA, allowing analysis of peripheral input from a defined orofacial region. The model will be useful in developing new strategies to treat chronic orofacial pain.Abbreviations NTS Nucleus tractus solitarius - ECu External cuneate nucleus - CFA Complete Freund's adjuvant - Cu Cuneate nucleus - Gr Gracile nucleus - IO Inferior olivary nucleus - LI Like-immunoreactivity - mlf Medial longitudinal fasciculus - Pa5 Paratrigeminal nucleus - PBS Phosphate-buffered saline - PG Parotid gland - Py Pyramidal tract - RVL Rostroventrolateral reticular nucleus - sol Solitary tract - sp5 Spinal trigeminal tract - Vi Subnucleus interpolaris of the spinal trigeminal nucleus - Vc Subnucleus caudalis of the spinal trigeminal nucleus - TB Tris buffer - TMJ Temporomandibular joint - Vi/Vc Trigeminal subnucleus interpolaris/subnucleus caudalis |
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| Keywords: | Inflammation Trigeminal nerve Fos Parotid gland Pain |
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