RP-HPLC法同时测定心宁片中芍药苷、阿魏酸和芦丁的含量

目的建立同时测定心宁片中芍药苷、阿魏酸和芦丁含量的RP-HPLC法。方法采用RP-HPLC法,以甲醇-乙腈-质量分数为1%的冰醋酸水溶液(体积比为25∶3∶70)为流动相,色谱柱为Diamonsil C18(200 mm×4.6 mm,5μm),流速为1.0 mL.min-1,柱温为35℃,检测波长为230nm。结果芍药苷、阿魏酸和芦丁分别在51.02~510.20、1.08~10.80和19.04~190.40 mg.L-1内,质量浓度与峰面积呈良好的线性关系,相关系数分别为0.999 9、0.999 9和0.999 6。方法平均回收率(n=9)分别为100.5%(RSD=1.0%)、100.4%(RSD=0.8%)和100.4%(RSD=0.9%)。结论RP-HPLC法可为心宁片的质量控制提供依据。

Simultaneous determination of paeoniflorin, ferulic acid and rutin in Xinning tablets by RP-HPLC

Objective To develop a RP-HPLC method for simultaneous determination of paeoniflorin, ferulic acid and rutin in Xinning(Tradition Chinese medicines) tablet. Method The analysis was performed on a Diamonsil C18 column (200 mm×4.6 mm, 5 μm) with a mobile phase of methanol-acetonitrile-1 % acetic acid (25:3:70). The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃.The detection wavelength was set at 230 nm. Results The calibration curve was linear within the range of 51.02 -510.20 mg·L-1 (r =0.999 9) for paeoniflorin，1.08-10.80 mg·L-1 (r =0.999 9) for ferulic acid and 19.04-190.40 mg·L-1(r =0.999 6)for rutin, respectively.The recoveries(n =9) of paeoniflorin, ferulic acid and rutin were 100.5 ％ (RSD =1.0 ％), 100.4 ％(RSD =0.8 ％)and 100.4 ％(RSD =0.9％), respectively. Conclusion The method is simple, accurate and selective. It can be applied to determine paeoniflorin,ferulic acid and rutin in Xinning tablet.