Enzymatic determination of red cell creatine as an index of hemolysis |
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| Authors: | H Cramer H Dauwalder H Meier J P Colombo |
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| Institution: | Department of Clinical Chemistry, Inselspital, University of Berne, Switzerland. |
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| Abstract: | An enzymatic assay for creatine, depending on the creatine kinase reaction, has been modified for the determination of creatine in packed erythrocytes, using a centrifugal analyzer (COBAS BIO). The method is precise, sensitive and shows excellent accuracy in recovery experiments when compared to the diacetyl-alpha-naphthol method. The enzymatic red cell creatine correlates with the erythrocyte survival time determined with the radioactive chromium method. It can be used as a rapidly available parameter for the quantification of hemolytic processes. |
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