血清、脑脊液中S-100B在颅脑外伤损伤程度中的诊断意义

目的 探讨血清、脑脊液中S-100B与颅脑外伤(traumatic brain injury,TBI)损伤程度的关系.方法 选择45例TBI患者按格拉斯哥昏迷分级(GCS评分)分轻、中、重三组(病例组),采用酶联免疫吸附法(EHSA)测定TBI患者伤后第1、3、5、7天血清及脑脊液S-100B水平,并选择20例与病例组相匹配的疝或静脉曲张手术病人以及健康体检自愿者的血清、脑脊液作为对照组,分析血清及脑脊液S-100B水平与TBI损伤程度的相关性.结果 病例组血清、脑脊液S-100B水平较对照组明显升高(P ＜0.01；P ＜0.01)；TBI患者GCS评分与血清及脑脊液S-100B水平比较呈负相关(r=-0.893,P＜0.01；r=-0.947,P＜0.01)；血清、脑脊液S-100B在不同程度TBI间比较差异显著(P＜0.01)；病例组血清和CSF中S-100B水平比较呈正相关；结论 血清、脑脊液S-100B测定均可作为判断TBI程度的指标,且各有特点.

Diagnostic significance of S-100B protein in serum and cerebrospinal fluid in patients with TBI

Objective To investigate the relationship between the extent of the damage in traumatic brain injury(TBI)and S-100B protein levels in serum and cerebrospinal fluid (CSF).Methods 45 cases of TBI patients were classified into three cohorts according to the Glasgow Coma Scale (GCS):minor grade,moderate and severe(case group), S-100B protein levels in serum and CSF were measured with ELISA in these patients 1,3,5,7 days after the injury, and selected 20 cases in control group including operative patients with hernia or varicose vein and healthy volunteers, analyzed the relationship between S-100B levels in serum and CSF and the degree of TBI damage.Results Case groups have a significantly higher serum, CSF S-100B levels than control group (P<0.01;P<0.01);GCS in patients were negatively correlated with serum and CSF S-100B levels(r=-0.893,P<0.01;r=-0.947, P<0.01);Differences of S-100B levels in serum and CSF among minor grade,moderate and severe being significant (P<0.01);Positive correlation was found between S-100B levels in serum and in CSF in case groups;Conclusions S-100B assay in serum and CSF can indicate the extent of lesion of TBI both,but with various characteristics.