胰腺成体干细胞的体外培养和鉴定

目的:用目前常用的干细胞分离方法对正常SD大鼠的胰腺成体干细胞进行分离、培养和鉴定,为胰腺癌干细胞的进一步研究奠定基础.方法:选取正常SD大鼠胰腺用胶原酶P消化分离,并用低血清无糖培养基纯化胰腺细胞,以RT-PCR,免疫组化和Western blot方法鉴定胰腺成体干细胞标志物巢蛋白(nestin)并通过RT-PCR证明细胞中存在ABCG2(ATP-binding cassette superfamily G member 2).结果:成功分离到所需胰腺成体干细胞,并通过以上3种方法证明分离所得细胞表达巢蛋白.结论:以胶原酶P作为消化液,低血清无糖培养作为分离方法能够成功的从正常胰腺中分离得胰腺成体干细胞.

Culture and identification of pancreas adult stem cells

Objective:To isolate,culture and identify SD rat pancreas adult stem cells used the common isolation method with stem cells at present;establish a basis for further studies of pancreas cancer stem cells.Methods:We had chosen the normal SD rat pancreas digesting with collagen enzyme P.Use low serum and no glucose culture medium to purify pancreas cells.Western blot,RT-PCR and ICH to identify nestin in pancreas adult stem cells.We also proved that there was ABCG2(ATP-binding cassette superfamily G member 2)in...