不同小鼠品系对天花粉蛋白诱导免疫抑制易感性的差异

为了解不同品系小鼠对天花粉蛋白 (Tk )诱导的免疫抑制应答能力的差异 ,应用OVA特异性T细胞体外增殖抑制试验 ,发现 8种近交系小鼠对Tk相关免疫抑制的敏感性不同。根据抑制幅度 ,C5 7BL/ 6 (H 2 b)被判为高易感品系 (HS ,highsusceptible ) ,同属H 2 k 的C3H/He和AKR为低易感品系 (LS ,lowsusceptible)。Tk浓度为 5 0ng/ml时的T细胞增值抑制百分率对HS为 4 7 8%± 3 4 % ;对两个LS分别为 15 9%± 3 0 %和 18 9%± 3 5 % ,两组间差异显著 (P <0 0 0 1)。这一差异可持续出现在 5 0～ 2 0 0ng/ml的Tk剂量范围内。其余 5个品系 (BALB/c、 6 15、T739、A、DBA/ 2 )的抑制幅度介于HS和LS之间。这一结果有助于研究相应免疫抑制的基因调控。

The Differences of Susceptibility for the Trichosanthin-Induced Immunosuppression Among Mouse Inbred Strains

Based on a suppression assay for the OVA-specific T cell proliferation in vitro, eight mouse inbred strains were quantitatively determined on their susceptibilities for the Trichosanthin (Tk)-induced suppression. One strain, C57BL/6 (H-2 b), behaves as HS (high susceptible) with percentages of suppression of 47.8%±3.4% at a Tk concentration of 50 ng/ml. In contrast, the low susceptible strains (LS) C3H/He and AKR, both are H-2 k, only yield suppressions of 15.9%±3.0% and 18.9%±3.5%, respectively. There were significant differences (P<0.001) between HS and LS, which could be maintained under the Tk dosages ranging from 50～200 ng/ml. The susceptibilities for other five strains (BALB/c, 615, T739, A, DBA/2) were between HS and LS. The results we obtained are valuable for elucidating the mechanisms underlying the genetic control of the Tk-induced immunosuppression.