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靶向Survivin的siRNA联合吉西他滨抑制胰腺癌细胞增殖
引用本文:刘文松,燕海姣,秦仁义. 靶向Survivin的siRNA联合吉西他滨抑制胰腺癌细胞增殖[J]. 中华实验外科杂志, 2006, 26(1): 849-851. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.012
作者姓名:刘文松  燕海姣  秦仁义
作者单位:华中科技大学同济医学院附属同济医院普外科,武汉,430030;中山大学肿瘤防治中心;
基金项目:国家自然科学基金资助项目
摘    要:Objective To construct the siRNA eukaryotic expression vector targeting Survivin gene, and investigate the chemotherapy sensitivity of pancreatic cancer line Panc-1 treated by gemcitabine. Methods The siRNA eukaryotic expression vector targeting Survivin gene was constructed. Panc-1 cells were transfected with negative control vector or siRNA vector and selected by G418, and the cell growth curve was drawn. The expression of Survivin mRNA and protein was detected by RT-PCR and Western blot respectively. Panc-1 cells or transfected cells were treated with gemcitabine for 24 h,and then the growth inhibition rate was measured by MTT assay, and cell apeptosis rate was measured by flow cytometry. Results The result of endonuclease digestion and DNA sequencing revealed that the recombinant plasmid psiRNA-Survivin was constructed successfully. Survivin mRNA and protein levels were reduced by 79.2% and 83.6% respectively in stably transfected Panc-1 cells as compared with control group, and the cell growth curve was much smoother, and the growth inhibition rate [ ( 24.6±4.5 ) % / (38.7±5.2 ) % ] and apoptosis rate of these cells [ ( 16.7±2.5 ) %/( 26.8±3.4 ) % ] were significantly increased after treatment by gemcitabine (P < 0.05 ). Conclusion The constructed siRNA eukaryotie expression vector targeting Survivin could decrease the Survivin expression,inhibit the growth of Panc-1 cells significantly,and increase the chemotherapy sensitivity to gemcitabine.

关 键 词:胰腺肿瘤   

siRNA targeting Survivin coupling with gemcitabine suppressing proliferation of human pancreatic cancer cells
Abstract:
Keywords:SurvivinsiRNAPancreatic neoplasmsSurvivinsiRNA
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