舒肝颗粒对大鼠肝星状细胞的凋亡的影响

目的探讨舒肝颗粒作用于体外培养的大鼠肝星状细胞(hepatic stellate cell,HSC)其是否对大鼠肝星状细胞凋亡有影响,及其可能的作用机制。方法体外培养大鼠肝星状细胞株(HSC-T6),分实验组和对照组,实验组加入舒肝颗粒,分3个浓度组:4.2、2.8、1.4mg/ml,作用24h后,加入AnnexinV/FITC和碘化丙锭,流式细胞仪检测肝星状细胞的凋亡;加入CD95抗体,流式细胞仪检测CD95的表达;采用免疫细胞化学法检测Bax和Bcl-2的表达。结果实验组肝星状细胞凋亡率较对照组明显增高,且呈浓度依赖性,差异具有统计学意义(P<0.05);随着浓度增高,CD95、Bax的表达率增高,呈浓度依赖性,Bcl-2的表达,实验组与对照组差别无统计学意义,但Bax/bcl-2的比值随着药物浓度组的增加而升高。结论舒肝颗粒可诱导肝星状细胞的凋亡,且呈浓度依赖性,舒肝颗粒可能通过介导Fas系统、上调Bax/Bcl-2比率促肝星状细胞凋亡。

Shugan Particles of Rat Hepatic Stellate Cell Apoptosis

Objective Observe the effect of Shugan particles in cultured rat hepatic stellate cells,whether apoptosis in rat hepatic stellate cells affected,and its possible mechanism.Methods Cultured rat hepatic stellate cells(HSC-T6),divided into experimental and control groups,the experimental group to join Shugan particles,divided into three concentrations:4.2mg/ml,2.8mg/ml,1.4mg/ml.During the 24h,we analyze the apoptosis using flow cytometry after staining with Annexin V-FITC/PI and CD95.Then we detect the expression of Bax /Bcl-2 using immunohistochemical method.Results Apoptosis rate in experimental group was significantly higher than the control group,and dose-dependent manner,the difference was significant(P<0.05).As the concentration increased,CD95,Bax expression rate increased,Bcl-2 expression in the experimental group and control group no significant difference,but the ratio of Bax/bcl-2 group with the increase of drug concentration ratio increased(P<0.01).Conclusion Shugan Granule can induce apoptosis(P<0.05).Shugan granule may induce HSC apotosis by mediating fas system.Shugan granule could up-regulate the rat′s Bax/Bcl-2 to increase the sensitivity of apoptosis and induced HSC apoptosis.