人神经干细胞移植治疗大鼠脑创伤观察

目的:探讨人神经干细胞(NSCs)移植治疗大鼠脑创伤的可行性.方法:取孕14周流产胎儿脑组织,进行NSCs培养及鉴定,并应用Hoechest33258标记细胞.54只大鼠随机等分为假损伤组(A组)、治疗对照组(B组)及NSCs移植组(C组).采用自由落体撞击法制作大鼠脑创伤模型后,C组给予NSCs移植,B组给予生理盐水,A组不打击脑组织.3组分别于移植术后2 d及7 d进行大鼠行为学评分,分别于植术后2周和4周行Y迷宫试验测学习和记忆评分.移植后3 d、7 d脑组织切片荧光显微镜下观察移植细胞存活情况,移植后2周、4周行GFAP、NSE免疫组织化学染色.结果:NSCs移植后2 d行为学评分3组之间差异有统计学意义(P=0.00),B、C组间差异无统计学意义(P=0.09);移植后7 d行为学评分3组间差异有统计学意义(P=0.00),A、C组间差异无统计学意义(P=0.10);移植后2周学习评分和4周记忆评分3组间差异均有统计学意义(P=0.00),B、C组间差异均有统计学意义(P=0.00);C组脑组织GFAP、NSE染色阳性,可见标记的NSCs.结论:人NSCs植入大鼠创伤脑内后,可存活并和宿主组织融合在一起,促进大鼠行为学恢复,提高学习和记忆能力,并分化为神经元和神经胶质细胞.NSCs移植为治疗脑创伤提供了新的方法.

Therapeutic effect of human neural stem cell transplantation on rat traumatic brain injury

Aim: To investigate the therapeutic effects of human neural stem cells(NSCs) transplantion on rat traumatic brain injury. Methods: NSCs were derived from human fetal subventricular zone and labled with fluorescein Hoechest33258 before transplantation.A total of 54 rats were randomly divided into 3 groups: sham injury group(A),treatment control group(B),and NSCs transplantation group(C). After experimental traumatic brain injury,NSCs were injected into rat cortex of group C,and normal saline was given to rats in group B.The motor function scores of rats were assessed at 2 d and 7 d after transplantation,study and memory abilities were observed by Y maze test at 2 weeks and 4 weeks after transplantation. At different time points after transplantation the animals were sacrificed and their brains were examined by immunohistology using GFAP and NSE staining. Results: Significant differences were observed in motor function scores at 2 d after transplantation among 3 groups(P=0.00),and no difference was observed between group B and C(P=0.09).Significant differences were observed in motor function scores at 7 d after transplantation among 3 groups(P=0.00),and no difference was observed between group A and C(P=0.10). There were significant differences in study and memory abilities among 3 groups (P=0.00),and differences were observed between group B and C(P=0.00).Lebelling NSCs could be found in group C. Conclusion:Implanted human NSCs can survive, and migrate to injuried zone and differentiate in traumatic rat brain,and improve motor function scores and study and memory abilities.