| 大鼠心肌缺血再灌注损伤中TNF基因表达状况 |
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| 引用本文： | 张景云,宫雁冰,徐国锋. 大鼠心肌缺血再灌注损伤中TNF基因表达状况[J]. 中国校医, 2010, 24(1): 28-30 |
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| 作者姓名： | 张景云 宫雁冰 徐国锋 |
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| 作者单位： | 辽宁基础医学研究所,辽宁,沈阳,110101 |
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| 摘 要： | 目的检测在心肌缺血再灌注损伤中TNF的表达，并初步探讨其机制。方法采用Langendorff模型及大鼠离体心脏灌注模型，心肌组织缺血30min后、再灌注30、60、120min时，用原位杂交、免疫组化和酶联免疫法检测心肌细胞中TNFrnRNA、蛋白的表达。结果缺血再灌注实验组心肌组织中的TNF蛋白水平比对照组明显升高；实验组心肌细胞中的TNFmRNA和蛋白表达明显，染色呈强阳性或极强阳性（t=2．689，P〈0．05），并且呈阳性表达的细胞是心肌细胞。结论缺血再灌注可刺激心肌细胞大量表达TNF，并且心肌组织中的TNF主要由心肌细胞表达产生。
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| 关 键 词： | 再灌注损伤 心肌缺血 肿瘤坏死因子 大鼠 Wistar |
TNF expression in rats with myocardial ischemic reperfusion indury |
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| ZHANG Jing-yun,GONG Yan-bing,XU Guo-feng. TNF expression in rats with myocardial ischemic reperfusion indury[J]. Chinese Journal of School Doctor, 2010, 24(1): 28-30 |
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| Authors: | ZHANG Jing-yun GONG Yan-bing XU Guo-feng |
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| Affiliation: | ZHANG Jing-yun,GONG Yan-bing,XU Guo-feng.Department of Technology,Vocational Technology College of Liaoning University of Traditional Chinese Medicine,Shenyang 110101,Liaoning,China |
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| Abstract: | Objective To observe the expression of TNF in rats with myocardial ischemic reperfusion injury,and explore the biological characteristics of it.Methods Adult male Wistar rats were subjected to myocardial ischemic reperfusion,after the myocardial tissue ischemic 30 min,with reperfusion for 30 min,60 min,and 120 min.The mRNA and protein expressions of TNF in myocardial cells were detected by in situ hybridization,immunohistochemistry and ELESA.Results The mRNA and protein expressions of TNF in myocardial cell... |
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| Keywords: | Reperfusion Injury Myocardial Ischemia Tumor Necrosis Factors(TNF) Rat Wistar |
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