大鼠脊髓慢性损伤脊髓组织PLA2、PGE2的检测

目的检测大鼠脊髓慢性损伤脊髓组织磷脂酶A2（PLA2）的活性、前列腺素E2（PGE2）的含量。方法采用生化活性法测定PLA2的活性，采用放射免疫法测定PGE2的含量。结果（1）PLA2活性：脊髓受压后各模型组PLA2活性增高，其中以中压组增高最明显（与假手术组比较，P〈0．01）；而重压组、轻压组与假手术组比较，P〈0．05；（2）PGE2含量：中、重压模型组与假手术组相比有非常显著性差异（P〈0．01），轻压模型组与假手术组相比有显著性差异（P〈0．05），重压模型组与轻压模型组相比也有显著性差异（P〈0．05）。结论大鼠脊髓慢性损伤后会继发炎症反应，炎症加重了脊髓的继发性损伤。

Detection of PLA2 and PGE2 in Rat Spinal Cord with Local Chronic Compression Injury

Objective To detect the activity of PLA2 and the content of PGE2 at local chronic compres- sion injury in rat spinal cord. Methods Biochemical activity method was used to detect the activity of PLA2. Radio-immunity method was used to detect the content of PGE2. Results (1) The activity of PLA2: The activity of PLA2 in experimental groups was increased after spinal cord compression, especial in the moderate compression group. There was significant difference (P<0.01) of the activity of PLA2 between the moderate compression group and the Sham-operated group. There also existed significant different (P<0.05) of the activity of PLA2 between severe, medium compression group and Sham-operated group. (2) The content of PGE2: There was significant difference (P<0.01) of the activity of PGE2 between the moderate, severe compression groups and the Sham-operated group. There also existed significant dif- ferent (P<0.05) of the activity of PGE2 between the mold compression group and Sham-operated group. And there existed significant different (P<0.05) of the activity of PGE2 between the mold compression group and the severe compression group. Conclusion After the chronic injury in rat spinal cord the continual inflammatory reaction can be produced, and it can make the continual injury in spinal cord more serious.