氧化型低密度脂蛋白和血脂康对人脐静脉内皮细胞产生一氧化氮合酶和细胞间黏附分子-1的影响

目的 :观察人脐静脉内皮细胞 (HUVEC)在氧化型低密度脂蛋白 (ox LDL)作用下内皮源性一氧化氮合酶 (eNOS)和细胞间黏附分子 1(ICAM 1)的变化及血脂康的干预作用。方法 :体外培养HUVEC ,制备ox LDL。用不同浓度 (5 0、10 0、2 0 0mg/L)的ox LDL和血脂康 (0 .1、0 .2 g/L)分别作用于HUVEC。 2 4h后 ,测定各组和对照组的eNOS和ICAM 1的含量 ;用免疫组化技术和图像分析法观察eNOS表达的变化 ;用酶联免疫吸附法测定细胞表面表达的黏附分子。结果 :ox LDL能抑制HUVEC产生的NOS活性和增强HUVEC产生的I CAM 1含量 ,与对照组比较差异均有统计学意义 ,且随ox LDL浓度增加 ,NOS活性减低呈剂量依赖性效应。血脂康能刺激HUVEC产生的NOS活性和减少ICAM 1的产生 ,与对照组比较差异均有统计学意义。结论 :ox LDL使HUVEC的NOS活性下降及ICAM 1增强 ,这对动脉粥样硬化 (AS)的发生起一定的作用。血脂康使HU VEC的NOS活性增强并减少ICAM 1的产生 ,对防治AS的发生起一定的作用。

Effects of oxidized low density lipoprotein and xuezhikang on nitric oxide synthase activity and intercellular adhesion molecule-1 in cultured human umbilical vein endothelial cells

Objective:To investigate the effects of oxidized low density lipoprotein (ox-LDL) and xuezhikang on nitric oxide synthase (NOS) activity and intercellular adhesion molecule-1 (ICAM-1) in cultured human umbilical vein endothelial cells (HUVEC).Method:HUVECS were cultured in vitro. The HUVECs were subjected to different concentrations of ox-LDL (50, 100, 200 mg/L) and different concentrations of xuezhikang ( 0.1, 0.2 g/L) for 24 h. The level of NOS and ICAM-1 in the HUVECs was determined . The level of NOS activity was measured with immunohistochemistry technique and image analyzing method. The level of ICAM-1 was measured with cell-ELISA.Result:ox-LDL significantly decreases NOS activity in a dose dependent manner and increases ICAM-1 expression in HUVEC. Xuezhikang increases NOS activity and decreases ICAM-1 expression in HUVEC.Conclusion:ox-LDL decreases NOS activity and increases ICAM-1 exprossion in HUVEC. Thus ox-LDL may play an important role in the genesis and development of atherosclorosis.Xuezhikang increases NOS activity and dcereases ICAM-1 expression. So xuezhikang may play an important role in preventing the genesis and development of atherosclerosis.