肝细胞癌组织中信号素3F 蛋白表达变化及其与患者预后的关系

目的：探讨肝细胞癌（HCC）组织中信号素3F（SEMA3F）蛋白表达与患者预后的关系。方法采用Western blot法检测32例HCC患者手术切除的HCC组织（ HCC组）和相应癌旁肝组织（对照组）标本中的SEMA3F蛋白表达，分析SEMA3F蛋白表达水平与HCC临床病理特征、复发转移及预后的关系。结果 HCC组及对照组SEMA3F蛋白表达阳性率分别为84％、97％，HCC组SEMA3F蛋白表达量低于对照组（P＜0．05）。肿瘤有包膜者SEMA3F蛋白表达量高于无包膜者，肿瘤为单个结节者表达量高于多个结节者（P均＜0．05）。 HCC组有、无肝硬化者的SEMA3F蛋白表达量差异无统计学意义。15例SEMA3F蛋白低表达者复发率显著高于17例高表达者，生存时间短于高表达者（P均＜0．05）。结论 SEMA3F可能在抑制HCC的侵袭转移中起重要作用。癌组织中SE-MA3F表达水平有助于判断HCC患者的预后。

Expression changes of SEMA3F in hepatocellular carcinoma and its relationship with clinical prognosis

Objective To investigate the expression changes of Semaphorin 3F （ SEMA3F） protein in hepatocellular carcinoma （ HCC） and its relationship with the clinical prognosis .Methods Western blotting was carried out in 32 cases of HCC samples surgically dissected to detect the expression of SEMA 3F protein in both HCC tissues （ HCC group） and pericarcinomatous liver tissues （control group）.Tthe relationship between the SEMA3F protein expression changes and clinicopathological features , recurrence/metastasis and clinical prognosis of HCC was analyzed .Results The SEMA3F positive expression rates in the HCC and control groups were 84% and 97%, respectively （P〉0.05）.Western blotting showed that expression level of SEMA3F in HCC group was lower than that in the control group （P〈0.05）.The expres-sion level of SEMA3F in tumor-capsulation was higher than that of tumor-without capsulation, and the expression level of SEMA3F in tumor-single nodular was higher than tumor-multiple nodular （all P〈0.05）.The expression level of SEMA3F between patients with liver cirrhosis and patients without liver cirrhosis in the HCC group had on significant difference （ P〉0.05）.The recurrence/metastatic rate of 15 cases of low expression was higher than that of 17 cases of high expression and the survival time was shorter than that of the high low expression group （all P〈0.05）.Conclusion SEMA3F may play an important role in the inhibition of invasion and metastasis of HCC and the expression level of SEMA 3F is helpful to judge the prognosis of patients with HCC .