| Purification of Fibronectin in Human Plasma and the Preparation of Its Antiserum |
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| 引用本文： | 郭河信,李进. Purification of Fibronectin in Human Plasma and the Preparation of Its Antiserum[J]. 中国人民解放军军医大学学报, 1989, 0(2) |
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| 作者姓名： | 郭河信 李进 |
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| 作者单位： | Department of Histology and Embryology First Military Medical University，Guangzhou，Department of Histology and Embryology，First Military Medical University，Guangzhou |
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| 摘 要： | The techniquc of purification of plasma fibroncctin and preparation of its antiserum is re-ported in this paper.Blood plasma was passed through an affinity column of gclatin-Sepharose 4B,fol-lowed by passing throughia column of Scphadex G200.Fibronectin with high purity was obtained.The prepare fibronectin serum.It was found that there were not only specific immune reactions be-tween the anti-human fibronectin serum and hyman fibronectins,but also cross reactions between theanti-serum and the fibronectin from other animals.The serum can be used for the study ofimmunohistochernistry of the fibronectin.
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Purification of Fibronectin in Human Plasma and the Preparation of Its Antiserum |
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| Guo Hexin Li Jin. Purification of Fibronectin in Human Plasma and the Preparation of Its Antiserum[J]. Journal of Medical Colleges of PLA(China), 1989, 0(2) |
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| Authors: | Guo Hexin Li Jin |
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| Affiliation: | Guo Hexin Li Jin Department of Histology and Embryology,First Military Medical University,Guangzhou |
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| Abstract: | The techniquc of purification of plasma fibroncctin and preparation of its antiserum is re- ported in this paper.Blood plasma was passed through an affinity column of gclatin-Sepharose 4B,fol- lowed by passing throughia column of Scphadex G200.Fibronectin with high purity was obtained. The prepare fibronectin serum.It was found that there were not only specific immune reactions be- tween the anti-human fibronectin serum and hyman fibronectins,but also cross reactions between the anti-serum and the fibronectin from other animals.The serum can be used for the study of immunohistochernistry of the fibronectin. |
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| Keywords: | fibronectin antigcn purification antiscrum preparation immunity |
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