外周血CD4~+T淋巴细胞表面CCR5受体表达水平与HIV感染关系的研究

目的研究人外周血中CD4+T淋巴细胞表面趋化因子受体5(chemokine receptor5,CCR5)的表达,比较健康人群、HIV急性期患者和HIV慢性期患者之间CCR5表达的差异,明确CCR5表达在正常人和HIV感染者之间的差异,以及CCR5表达与HIV病情进展的关系,以明确CCR5在HIV患者外周血CD4+T淋巴细胞表面表达的意义,并为CCR5拮抗剂在HIV临床治疗中的作用提供临床标本依据。方法收集来自首都医科大学附属北京佑安医院的260例HIV感染者和60例健康人的外周血标本,其中急性期感染者98例、慢性期感染者162例,利用淋巴细胞分离液分离外周血中PBMC,在显微镜下进行淋巴细胞计数,应用直接荧光标记的单克隆抗体对外周血单个核细胞(peripheral blood mononuclear cells,PBMC)表面CD4分子及CCR5进行染色,利用流式细胞术分析CD4+T淋巴细胞表面CCR5表达;对流式细胞术所得检测结果进行统计学分析。结果检测结果表明:(1)HIV急性期患者和HIV慢性期患者外周血中CD4+T淋巴细胞表面CCR5的表达显著高于正常人,差异有统计学意义(P<0.01);(2)HIV慢性期患者外周血中CD4+T淋巴细胞表面CCR5的表达显著高于HIV急性期患者,差异有统计学意义(P<0.01)。结论此项研究表明外周血中CD4+T淋巴细胞表面CCR5表达的差异与HIV的易感性、疾病进展情况密切相关。外周血中CD4+T淋巴细胞表面CCR5表达的检测,将对HIV患者的早期诊断、治疗和判断预后具有一定的临床意义。

Relationship between CCR5 Receptor Expression Levels of CD4+T Lymphocytes in Peripheral Blood and HIV Infection

Objective To study the expression levels of human peripheral blood CD4⁺T cell surface chemokine receptor 5(CCR5),and study the difference among healthy people,HIV patients in chronic phase and HIV patients in acute phase of the expression levels of CCR5,to clarify the differences of CCR5 expression levels between HIV patients and the normal individuals,and to show the relationship of CCR5 expression levels and the progression of HIV disease,and also to clarify the significance of CCR5 expression levels in peripheral blood CD4⁺T lymphocytes of HIV patients.Methods Blood specimens were collected from 260 cases with HIV infection and 60 healthy persons from Beijing Youan Hospital,Capital Medical University.Of the 260 HIV infected cases,98 had acute HIV infection,162 had chronic HIV infection.The peripheral blood mononuclear cells(PBMC) surface CD4 and CCR5 molecules were stained by direct fluorescent monoclonal antibody,and flow cytometry was used to analyze the expression levels of CD4⁺T cell surface CCR5.The results of the flow cytometry test were analyzed by using two sample t-test.Results (1) The peripheral blood CD4⁺T lymphocyte surface CCR5 expression levels of HIV patients in acute phase and chronic phase were significantly higher than those of normal controls(P<0.01);(2) The expression level of peripheral blood CD4⁺T lymphocytes surface CCR5 of the HIV patients in chronic phase was higher than that in HIV patients in acute phase(P<0.01).Conclusion Expression levels of peripheral blood CD4⁺T lymphocytes surface CCR5 may be closely related to the susceptibility to HIV infection and the disease progression.Determination of peripheral blood CD4⁺T cell surface CCR5 expression levels may have certain important clinical significance in early diagnosis,treatment and prognosis of HIV infected patients.