人类子宫颈癌基因、p16及人乳头瘤病毒16／18在子宫颈癌中表达的意义

 目的　研究人类子宫颈癌基因（HCCR）、p16在子宫颈上皮内瘤变（CIN）及子宫颈癌中的表达, 探讨其与人乳头瘤病毒（HPV）16／18感染的相关性及其临床意义。方法　采用免疫组织化学SP法检测28例慢性子宫颈炎，62例CINⅠ~Ⅱ级、49例CINⅢ级及52例子宫颈癌组织中p16、HCCR蛋白的表达，并采用原位杂交法检测其HPV16／18的表达。结果　HCCR在慢性子宫颈炎、CINⅠ~Ⅱ级、CINⅢ级和子宫颈癌组的阳性表达率分别为3.57 %（1／28）、35.48 %（22／62）、61.22 %（30／49）和88.46 %（46／52），各组间两两比较，差异有统计学意义（P＜0.05）。p16蛋白的阳性表达率从慢性子宫颈炎、CINⅠ~Ⅱ级、CIN Ⅲ级到子宫颈癌组逐渐增加，分别为7.14 %（2／28）、33.87 %（21／62）、65.30 %（32／49）和92.31 %（48／52），各组间两两比较差异均有统计学意义（P＜0.05）；HPV16／18在慢性子宫颈炎、CINⅠ~Ⅱ级、CINⅢ级和子宫颈癌组的阳性率分别为10.71 %（3／28）、40.32 %（25／62）、69.39 %（34／49）和84.62 %（44／52），除子宫颈癌与CINⅢ级比较差异无统计学意义外（P＝0.115），其余组间两两比较，差异有统计学意义（P＜0.01）。p16、HCCR的表达与HPV16／18感染呈正相关（P＜0.01）。结论　HPV感染可能通过影响p16及HCCR蛋白的过表达与子宫颈癌及其癌前病变的发生、发展密切相关，三者联合检测对子宫颈癌及癌前病变的筛查和预防具有重要意义。

Expression and significance of human cervical cancer oncogene,p16 and human paplilloma virus16/18 in cervical carcinoma

Objective To investigate the expression of human cervical cancer oncogene(HCCR)and p16 protein in cervical intraepithelial neoplasia(CIN)and cervical carcinoma,and explore the correlations between their expressions and HPV 16/18 infection and their significance. Methods The expressions of HCCR,p16 protein were detected by SP immunohistochemistry method and the expression of HPV 16/18 was detected in situ hybridization in 28 cages of chronic cervicitis.62 cases of CIN I-Ⅱ.49 cases of CIN Ⅲ and 52 cases of cervical carcinoma.Results The positive rates of HCCR in chronic cervicitis,CIN I-Ⅱ,CIN Ⅲ and cervical carcinoma were 3.57%(1/28), 35.48%(22,62), 61.22%(30/49) and 88.46%(46/521,respectively, and the difiefences were statistically significant fP<0.05)while compared between each two groups.The positive expression rates of p16 increased gradually,which were 7.14%(2/28)in chronic cervicitis,33.87%(21/62)in CIN Ⅰ-Ⅱ,65.30%(32/49)in CIN Ⅲ and 92.31%(48/52)in cervical carcinoma,when compared between each two groups,the differences were statistically significant(P<0.05).The positive rates of HPV16/18 in chronic cervicitis,CIN Ⅰ-Ⅱ.CIN Ⅲ and cervical carcinoma were 10.71% (3/28),40.32%(25/62),69.39%(34/49)and 84.62%(44/52),respectively,except that comparison between CIN Ⅲ and cervical carcinoma was not statistically significant (P=0.115).the differences were statistically significant between any other two groups (P<0.05).The expressions of HCCR and p16 were positively correlated with HPV16/18 (P<0.01).Conclusion HPV infection may affect on the overexpression of HCCR and p16 protein,which is highly correlation with the development of cervical carcinoma and precancerous lesions. Combined detection of them have important significance in screening and preventing cervical carcinoma and precancerous lesions.