实验性原位性肾炎的药物防治研究

本文用抑制TxA_2合成酶的药物——苄基咪唑(BIm)和抑制血小板聚集的活血化瘀中药藏红花、毛冬青甲素和丹参对实验性原位性肾炎模型进行了干扰,结果显示BIm,藏红花和毛冬青甲素组动物的尿蛋白显著低于对照组,肾小球中的免疫复合物吸收加速,病理组织损害亦较对照组有显著的改善,尿毒症的死亡率较对照组减少。但丹参组和对照组比较则无显著性差别。结论;藏红花、毛冬青甲素和BIm对实验性原位性肾炎有治疗作用。提示血小板和TxA_2在原位性肾炎发病机理和病情发展中起着重要的致病作用。

Experimental Study on Drug Prophylaxis and Treatment of in Situ Immune Complex Glomerulonephritis in Rabbits

The effects of a selective thromboxane A 2 synthetase inhibitor, i-benzylimidazole (BIm) and antiplatelet agents, traditional Chinese herbs Crocus Sativus (CS), Ilexonin A (IA), and Radix Salviae Miltiorrhizae (RSM) on in situ immune complex glomerulonephritis (GN) in rabbits were evaluated. Cationic bovine serum albumin (c-BSA) (PI =8.7) was injected intravenously to induce membranous nephropathy in immunized rabbits for Q weeks. 40 animals were divided into 5 groups, 4 groups treated with agents and one untreated (control). After 2 week dosage of c-BSA injection, the agents were administered for 10 weeks repectiv-ely. It was showed that BIm, CS and IA could significantly reduce the amount of urinary protein. As the c-BSA nephritis developed, serum creatinine (Cr), serum urea nitrogen (Sun), and creatinine clearance (Ccr) v/ere worsen in control, but the administration of BIm to c-BSA nephritis had beneficial effects on Ccr. Histological examination by immunofluorescence, light microscopy and electron microscopy revealed glomerular deposition of IgG and Cs was less intense after treatment; glomerular capillary wall-thickening, mesangial cell proliferation and mesangial matrix increasing were milder in treated animals; BIm-treated group also showed significantly less formation of glomerular epithelial crescent. The rate of uremic death in animals treated with BIm, CS and IA was significantly lower than that in nontreated animals. In our study, it showed BIm, CS and IA couldn't effectively prevent the immune complex deposition in GN induced by c-BSA, but it is suggested that BIm, CS and IA could be more effective than RSM against the in situ immune complex GN in rabbits.