缺氧复合氰化钠中毒对大鼠BALF和肺组织磷脂含量的影响

目的研究缺氧合并氰化钠(NaCN)中毒对大鼠肺支气管灌洗液(BALF)和肺组织中磷脂含量的影响。方法雄性SD大鼠72只,随机分为平原实验组和高原实验组。高原实验组动物置于低压舱(4000m,61kPa,舱内温度20±3℃)内,平原实验组置于普通试验室(海拔308m,大气压97.7kPa,室温20±3℃)内3d后进行实验。每组设0、0.5、1、2、4、6h等6个观测点,0h时点大鼠不注射NaCN,其余大鼠腹腔注射NaCN(3.6mg/kg),于注射后0、0.5、1、2、4h和6h时点放血处死动物,取全肺,分别检测肺/体比值,肺干/湿重比,制取BALF及肺组织匀浆,定磷法测总磷脂(TPL)含量。结果高原实验组部分大鼠鼻腔有少量泡沫状血性分泌物流出,注射后1h内3只动物中毒死亡。平原实验组大鼠NaCN中毒后,肺/体比值、肺干/湿重比、BALF中TPL含量显著增加(P<0.05),而肺组织中TPL含量明显降低(P<0.05)。高原实验组大鼠NaCN中毒后,肺/体比值、肺干/湿重比、BALF中TPL含量增加更显著(P<0.05),肺组织中TPL含量降低更明显(P<0.05)。结论缺氧和氰化物中毒可能对肺组织存在联合损伤效应。

Effects of combined hypoxia and NaCN intoxication on the phaspholipids in rats BALF and lung tissues

Objective To study the effects of combined hypoxia and sodium cyanide(NaCN)intoxication on the phospholipids in rats' BALF and lung tissues.Methods Seventy two male SD rats were randomly divided into 2 groups:the hypobaric hypoxia group(HH)in 4000m altitude and the normoxia non-hypoxia group(NH)in 308m altitude.The animals for the experiment of hypoxia were processed in a artificial hypobaric chamber to simulate the designated high altitude hypoxia(4000m,61kPa).Sodium cyanide(NaCN)was injected subcutaneously to the rats in the both groups in a dosage of 3.6mg/kg.The animals were killed immediately at the time points of 0,0.5,1.0,2.0,4.0 and 6.0h.Bronchoalveolar lavage fluid(BALF)and lung tissues were prepared at the same time.The weight ratios of lung/body and dry/wet of lung tissue were measured and the phospholipids content was determined with a biochemistry method.Results Non-hypoxia and hypobaric hypoxia produced a significant increasing effect on the phospholipids content in the rats BALF and the weight ratios of lung/body and dry/wet of lung tissue.Phospholipids content in the lung tissue of the rats,however,was obviously decreased.Acute hypobaric hypoxia and sodium cyanide intoxication produced a more significant increasing effect on the phospholipids content in the rats BALF and the weight ratios of lung/body and dry/wet of lung tissue.All the changes of the parameters mentioned above were further increased by the treatment of sodium cyanide injection.Conclusion Hypoxia and sodium cyanide intoxication may induce a combined injury effect on animal lung tissues.