Perinatal expression of heat-shock protein 27 in brain regions and nonneural tissues of the piglet |
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Authors: | Jean Claude David Jacques Landry Jean François Grongnet |
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Institution: | Laboratoire de Sciences Animales, Ecole Nationale Supérieure Agronomique, Rennes, France. |
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Abstract: | Important stressful events occur at birth or within the few hours that follow this event. To unravel a possible involvement
of stress proteins, it is important to determine their levels of expression. Expression of HSP27 protein was determined by
Western blotting in several nonneural tissues and in brain regions of the developing pig (fetal to adult). Maximum levels
of expression were observed in heart, liver, and lung. In kidney, the expression was reduced during the first hours of life.
Brain parts included whole brain, cerebellum, cortex, hippocampus, hypothalamus, and striatum. HSP27 immunogenicity was observed
in all the brain regions studied. In whole brain, cerebellum, cortex, hippocampus, and hypothalamus extracts, the levels of
HSP27 were decreased during the first hours of age (4 h to 2 d). In striatum, levels of expression were very low—if detectable—during
the early postnatal days of life. Changes in environmental parameters, like temperature and/or hypoxia can be possibly related
to differential expressions of HSPs, which can result in severe adverse developmental outcomes. The results are discussed
in terms of using the newborn piglet as a model to study different forms of stress on the heat-shock protein postnatal expression. |
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Keywords: | HSP27 perinatal development brain non-neural tissues piglet |
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