建立SCID鼠增生性瘢痕模型的实验研究

目的 建立联合重症免疫缺陷鼠(SCID)增生性瘢痕模型并探究其可行性。方法 将人类刃厚皮片移植至SCID鼠背部(n=20),术后2周、4周时取材,对移植物进行HE和Masson染色观察,比色法测定羟脯胺酸含量(术后4周),RT-PCR测定组织中转化生长因子(TGF-β1)及结缔组织生长因子(CTGF)的表达水平。结果 通过该模型得到了红褐色、高出皮面、质韧的类瘢痕组织,组织学检测发现该组织具有真皮层增厚、皮肤附件结构改变、血管增生、胶原束细小及排列紊乱等增生性瘢痕的特点;术后4周,实验组羟脯胺酸含量较对照组明显增加(P<0.05);术后2周、4周时,实验组TGF-β1、CTGF表达水平均较对照组明显上调(P<0.01)。结论 该模型与人类增生性瘢痕有很多相似性,并且操作简单,可控性强,有望在增生性瘢痕的进一步研究中发挥作用。

Experimental Study of Establishing Hypertrophic Scar Model on SCID Mice

Objective To establish hypertrophic scar(HS)model on severe combined immunodeficiency(SCID)mice and investigate the feasibility. Methods Human split-thickness skin graft was transplanted onto the dorsum of SCID mice, the harvested xenograft was then assessed and given biopsy for histology by hematoxylin & eosin (HE) or Masson's trichrome staining 2 and 4 weeks after operation, and hydroxyproline quantification by colorimetry 4 weeks after operation. RT-PCR analysis was also performed to identify mRNA levels of transforming growth factor-β1(TGF-β1)and connective tissue growth factor (CTGF)2 and 4 weeks after operation. Results The xenograft showed a characteristic of human HS including a red, firm, elevated appearance, and dermal thickness, changed adnexal structures, hypervascularization, thinner collagen fibrils and disorganization of them. The content of hydroxyproline in experimental group increased significantly compared to the control group 4 weeks after operation(P<0.05).Moreover,RT-PCR showed significantly elevated mRNA levels for both TGF-β1 and CTGF in experimental group 2 and 4 weeks after operation (P<0.01). Conclusion This animal model has intrinsic properties that closely resemble HS formation as seen in humans, which shares additional merits of simple operation and flexible controllability and therefore can be expected to play a critic role in the research of human HS.