慢性不可预知性应激刺激后大鼠行为学的改变

目的:建立长期慢性不可预知性应激刺激大鼠动物模型,观察大鼠在应激后行为学的改变。方法:雄性SD大鼠20只,5周龄,饲养3周后随机分为实验组和对照组,每组10只。实验组进行为期5周的随机应激刺激,建立慢性不可预知性应激动物模型,对照组不施加任何刺激,正常饲养。两组大鼠每周都要进行体质量测量、矿场行为学测验以及糖水偏好测试,将两组的测试结果进行比较。结果:两组大鼠在体质量方面无明显差异,而矿场实验,实验组与对照组从第14天就开始出现显著差异(t=-2.420,P=0.016);糖水测验两组出现显著差异则出现在21天时(t=5.854,P=0.000)。结论:大鼠的行为活动在长期慢性不可预知性应激刺激后明显改变,说明大鼠长期处在应激刺激下,正常的行为活动会出现障碍。

The Changes of Behaviors in Rats Induced by Chronic Unpredictable Stress in Rats

Objective:To observe the changes of behavior of rats in stress state induced by chronic unpredictable stress experiments.Methods:When twenty 5-week-old male SD rats had been feed for 3weeks were randomly di-vided into 2 groups,the control group and the experimental group.Each group had ten rats.The experimental group were suffered chronic unpredictable stress stimulations everyday until the fifth week.The control group drank and ate normally everyday.2 groups rats were weighted every week and evaluated by open-field box and sucrose preference test,then compared the scores of 2 groups.Results:There was no significant difference between control group and ex-perimental group in weight.But there was significant difference between experimental group and control group in the score of the open-field from the fourteenth day(t=-2.420,P<0.05);There were significant difference between ex-perimental group and control group in the scores of sucrose preference test from the twenty-first day(t=5.854,P<0.05).Conclusion:Long-term chronic unpredictable stress stimulation can affect the general body state of rats.