| 乌头类生物碱对bcl-2基因表达的影响 |
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| 引用本文： | 饶朝龙,彭成.乌头类生物碱对bcl-2基因表达的影响[J].卫生毒理学杂志,2010(2). |
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| 作者姓名： | 饶朝龙 彭成 |
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| 作者单位： | 成都中医药大学药学院药理学教研室; |
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| 基金项目： | 国家重点基础研究发展计划(2006CB504705); 国家自然科学基金重点项目(30230410) |
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| 摘 要： | 目的研究乌头类生物碱对大鼠视网膜神经细胞细胞增殖周期和bcl-2基因表达的影响,探讨乌头类中药神经毒性的作用机制。方法体外培养大鼠视网膜神经细胞,分别以0.5%的乌头碱、新乌头碱和次乌头碱作用细胞5min后,流式细胞仪检测。结果与阴性对照组比较,乌头碱作用组G2/M期细胞比例从2.3%增加到19.5%,细胞增殖活动被抑制;而bcl-2基因表达量显著增加,表达荧光均值从0.78增加到1.45。新乌头碱和次乌头碱作用组结果与乌头碱类似。结论在本试验条件下,bcl-2基因可能在乌头类生物碱的神经毒性作用机制中具有重要意义。
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| 关 键 词： | 乌头类生物碱 视网膜神经细胞 bcl-2基因 神经毒性 |
The effect of aconitic alkaloid on bcl-2 gene expression |
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| RAO Chao-long,PENG Cheng.The effect of aconitic alkaloid on bcl-2 gene expression[J].Journal of Health Toxicology,2010(2). |
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| Authors: | RAO Chao-long PENG Cheng |
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| Institution: | RAO Chao-long,PENG Cheng (College of Chinese Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu Sichuan 610075,China) |
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| Abstract: | Objective To study the effect of aconitic alkaloid on cell cycle and bcl-2 gene expression of rat retina neuron,and explore the mechanism of aconitic neurotoxicity. Methods Rat retina neuron was cultivated in vitro,and treated with aconitine,mesaconitine and hypaconitine for 5 minutes respectively,then determined with Flow Cytometer. Results Compared with negative control,the proportion of G2 /M cell in the group treated with aconitine increased from 2. 3% to 19. 5% , which meant that cell proliferation had... |
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| Keywords: | Aconitic alkaloid Retina neuron Bcl-2 gene Neurotoxicity |
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