636例血标本的病原菌种类分布与耐药性研究

目的 了解本院血培养分离到的病原菌菌种及对抗生素的耐药性.方法 利用Bact/Alert3D全自动血培养仪及其配套的血培养瓶对636份标本进行血培养,阳性血培养转种后用MicroScan A/s-4细菌鉴定及药敏测试仪进行测试.结果 636份血培养标本中,检出病原菌65株(10.22%),革兰阳性球菌占40%,革兰阴性杆菌占55.4%,真菌占4.6%,葡萄球菌属和肠杆菌科的细菌是本院菌血症和(或)败血症的主要病原菌.革兰阳性球菌中未发现对万古霉素耐药的菌株,革兰阴性杆菌对亚胺培南有高度敏感性,但对常用抗生素有较普遍的耐药性.结论 血培养检出菌种复杂,耐药率高,应加强血培养中致病菌及其对抗生素的耐药性监测.

Analysis on the result of blood culture and drug resistance of pathogens isolated from 636 blood samples

Objective To explore the isolated bacteria and their drug resistance from blood culture samples in our hospital.Methods 636 blood samples were cultivated by Bact/Alert3D Automated Blood Culture System and appropriative culture bottles.The positive blood cultures were reinoculated. Then species identification and drug sensitivity tests were performed by the MicroScan A/s-4.Results Among the 636 blood samples, 65 strains of phthogens were isolated (10.22%).40% were gram-positive cocci, 55.4% were gram-negative bacilli, and 4.6% were fungi.Staphylococcus and enterobactericaeae were main pathogens in bacteremia and (or) septicaemia in our hospital . No strains resistant to vancomycin was found in gram-positive cocci. Gram-negative bacilli were highly sensitive to imipenem.But they were resistant commonly to antibiotics used often.Conclusion The kinds of isolated bacteria were complex from the positive blood cultures and their drug-resistance rates were high. More attention should be paid to the detection and surveillance of bacterial resistance in blood culture.