对乙型肝炎病毒联合检测的评价

目的 研究乙型肝炎(下称乙肝)病毒(HBV)血清标志物、前S2抗原检出情况与HBV-DNA结果之间的关系.方法 对472例临床样本采用酶联免疫吸附试验(ELISA)分别进行HBV血清标志物5项、前S2抗原的检测,同时运用荧光定量聚合酶链反应法检测HBV-DNA的含量.结果 HBV表面抗原(HBsAg)、HBV e抗原、HBV核心抗原抗体(抗-HBc)阳性组前S2抗原阳性检出率为98.7%和HBV-DNA阳性检出率为84.4%均显著高于HBsAg、抗e抗原抗体、抗HBc阳性组(P＜0.01);159例血清HBV-DNA呈阴性组中前S2抗原检测出67例阳性;130例前S2抗原检测呈阴性组中血清HBV-DNA检出38例阳性.结论 前S2抗原、HBV-DNA及HBV血清标志物检测都可对HBV的复制情况进行评估,但并不同步,需联合应用方可对HBV感染、复制情况作出较全面的评估.

Clinical signification of combined detection of HBV markers, pre-S2 antigen and HBV-DNA in diagnosis of hepatitis B virus infection

Objective To investigate the relationship among HBV markers,pre-S2 antigen and HBV-DNA.Methods 472 cases of clinical species were collected.ELISA was applied to detecting HBV markers and pre-S2 antigen,while FQ-PCR was applied to detecting HBV-DNA content.Results The positive rate of pre-S2 antigen and HBV-DNA was 98.7% and 84.4% in HBsAg,HBeAg and anti-HBc positive group respectively,higher significantly than that of HBsAg,anti-HBe and anti-HBc positive group(P<0.01).In serum HBV-DNA negative group,67 cases were pre-S2 antigen positive;while in pre-S2 antigen negative group,38 cases were serum HBV-DNA positive.Conclusion HBV markers,pre-S2 antigen or HBV-DNA can evaluate HBV duplication,but not simultaneous.Combined detection of the three methods should be applied to assessing HBV infection and duplication totally.