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两种方法对比检测孕妇血型免疫性抗体
引用本文:王辉,彭献.两种方法对比检测孕妇血型免疫性抗体[J].检验医学与临床,2007,4(7):615-616.
作者姓名:王辉  彭献
作者单位:广东省深圳市第九人民医院检验科,518116
摘    要:目的 分析比较凝胶微柱法与传统经典抗人球蛋白法测定O型孕妇血清中血型免疫性抗体效价的方法学差异.方法 采用微柱凝集抗人球方法和经典抗人球蛋白试验平行检测77例与丈夫ABO血型不合的O型孕妇血清IgG抗A(B)水平,并对两种方法的结果进行方法学评价.结果 分析测定77例样本,经统计学处理,两种方法之间的差异有统计学意义(P<0.01).结论 凝胶微柱法比试管间接抗人球法具有更高的灵敏度,且实验操作简便,快速,更适合于实验室内的广泛应用.

关 键 词:血型免疫性抗体  微柱凝集技术  经典抗人球蛋白法  方法对比  平行检测  孕妇  血型免疫性抗体  antigens  blood  group  antibodies  test  tube  conventional  assay  microcolumn  应用  实验室  快速  实验操作  灵敏度  抗人球法  试管  统计学意义
文章编号:1672-9455(2007)07-615-02
修稿时间:2007-05-21

Comparison of gel microcolumn assay and conventional tube test for IgG antibodies against blood group antigens
WANG Hui,PENG Xian.Comparison of gel microcolumn assay and conventional tube test for IgG antibodies against blood group antigens[J].Laboratory Medicine and Clinic,2007,4(7):615-616.
Authors:WANG Hui  PENG Xian
Institution:WANG Hui PENG Xian No 9 People's Hospital of Shenzhen Shenzhen Guangdong 518116 China
Abstract:Objective To compare the gel microeolumn assay and the conventional tube test for IgG anti A(B) titration.Methods Seventy-seven blood samples of"O"group pregnant women,whose husbands were of non"O"group,were detected simultaneously by the conventional tube test and the gel microcolumn assay.The results were evaluated.Results Through the analysis of the results in 77 blood samples,IgG anti A(B)titration in the gel mi- crocolumn assay showed significantly higher than that in the conventional tube test in all samples studied(P<0.01). Conclusion The gel microcolumn assay is superior to the conventional tube test.It has the further advantage of standardization of the test,shorter incubation time,and no washing procedure.
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