| C反应蛋白抑制血小板内皮型一氧化氮合酶活性 |
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| 引用本文： | 王稳,徐标.C反应蛋白抑制血小板内皮型一氧化氮合酶活性[J].高血压杂志,2008(4). |
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| 作者姓名： | 王稳 徐标 |
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| 作者单位： | 南京大学医学院附属鼓楼医院心内科;江苏宜兴市人民医院心内科; |
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| 基金项目： | 江苏省自然科学基金(BK2004083); 国家自然科学基金(NO30170370) |
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| 摘 要： | 目的研究不同浓度的纯化重组人 C 反应蛋白(rhCRP)对正常人血小板内皮型一氧化氮合酶(eNOS)活性的影响。方法取健康人外周静脉血,用凝胶色谱柱法收集血小板,用同位素二步色谱法测定血小板eNOS 的活性。将收集的血小板和组织胺、N_ω硝基左旋精氨酸甲酯盐酸盐(L-NAME)、不同浓度纯化的 rhCRP-起孵育后测定血小板 eNOS 活性。结果组织胺可以明显增加血小板 eNOS 活性;eNOS 抑制剂 L-NAME 可以明显抑制 eNOS 活性以及用组织胺刺激后的血小板 eNOS 活性增加;rhCRP 明显抑制组织胺刺激后的 eNOS 活性,且这种抑制作用呈现显著的浓度依赖性。结论 rhCRP 对正常人血小板经组织胺诱导的 eNOS 活性有显著抑制作用,这种抑制作用一定范围内呈现显著的浓度依赖性。
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| 关 键 词： | C 反应蛋白 血小板 一氧化氮合酶 一氧化氮 |
Effects of C-reactive Protein on Platelet Endothelial Nitric Oxide Synthase Activity |
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| WANG Wen XU Biao De- partment of Cardiology,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing ,China.Effects of C-reactive Protein on Platelet Endothelial Nitric Oxide Synthase Activity[J].Chinese Journal of Hypertension,2008(4). |
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| Authors: | WANG Wen XU Biao De- partment of Cardiology The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing China |
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| Institution: | WANG Wen XU Biao De- partment of Cardiology,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China |
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| Abstract: | Objective To study the effect of recombinant human C-reactive protein (rhCRP) on platelet endo- thelial nitric oxide synthase (eNOS) activity.Methods Peripheral venous blood was collected and platelets were i- solated with gel-filtration chromatography and incubated with histamine.L-NAME and different concentrations of rhCRP(5,10,25,50,100 mg/L) were co-cultured with platelet for 30 minutes,eNOS activity was measured by production of ~3H L-citrulline from ~3 H-L-arginine.Results Platelet eNOS activity was s... |
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| Keywords: | C-reactive protein Platelet Nitric oxide synthase Nitric oxide |
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