| 人类染色体17p13.3位点新克隆基因HC56,HC71 和HC90在白血病细胞的表达 |
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| 引用本文： | 王嵘,波西雁,万大方,郁志,杜均祥,牧启田,张洹,顾建人.人类染色体17p13.3位点新克隆基因HC56,HC71 和HC90在白血病细胞的表达[J].中国实验血液学杂志,2002,10(5):419-422. |
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| 作者姓名： | 王嵘 波西雁 万大方 郁志 杜均祥 牧启田 张洹 顾建人 |
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| 作者单位： | 1. 暨南大学医学院血液病研究所,广州,510632
2. 上海市肿瘤研究所癌基因与相关基因国家重点实验室,上海,200032 |
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| 基金项目： | 教育部科学基金资助项目编号教技司[2 0 0 0 ] 156号 |
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| 摘 要： | 为研究人类染色体17p13．3位点新克隆的基因HC56，HC71和HC90在白血病细胞中的表达，应用同位素标记的、以βM2基因作为内参的半定量RT—PcR法，检测35例急性白血病和4株白血病细胞系的Hc56，Hc70和HC90基因的表达。结果显示，急性淋巴系白血病病人的HC90基因和T淋巴系白血病细胞系Jurkat细胞HC90基因表达水平降低。急性髓系白血病病人HC71基因表达水平降低。HC56基因表达在急性白血病和正常对照间未见显差异。结论：在人类白血病有HC70和HC90的基因表达异常。
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| 关 键 词： | 人类 染色体17p13．3位点 白血病细胞 基因表达 HC56 HC71 HC90 |
| 修稿时间： | 2001年9月10日 |
Expression of Novel Cloned Genes HC56, HC71 and HC90 Mapped on Human Chromosome 17p13.3 in Leukemic Cells |
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| WANG Rong,PO Xi Yan,WAN Da Fang ,YU Zhi,DU Jun Xiang,MU Qi Tian,ZHANG Yuan,Gu Jin Ren.Expression of Novel Cloned Genes HC56, HC71 and HC90 Mapped on Human Chromosome 17p13.3 in Leukemic Cells[J].Journal of Experimental Hematology,2002,10(5):419-422. |
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| Authors: | WANG Rong PO Xi Yan WAN Da Fang YU Zhi DU Jun Xiang MU Qi Tian ZHANG Yuan Gu Jin Ren |
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| Institution: | Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, China. rwang@iarc.fr |
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| Abstract: | In order to investigate expression of novel genes HC56, HC70 and HC90 mapped on chromosome 17p13.3 in human leukemic cells, HC56, HC71 and HC90 genes expression was examined in cells from 35 patients with acute leukemias and 4 leukemic cell lines by using semi-quantitative RT-PCR with incorporation of alpha(32)P dATP, betaM2 gene as endogenous control. The results showed that HC90 gene expression level was lower in patients with acute lymphocytic leukemia and T lymphocytic leukemic cell line Jurkat than that in normal control. Low-level HC71 gene expression was detected in acute myeloid leukemia. There was no expression difference in HC56 between leukemic cells and normal blood cells. It was concluded that both HC70 and HC90 genes were aberrantly expressed in human leukemic cells. |
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| Keywords: | chromosome 17p13 3 gene expression leukemic cell HC56 HC71 HC90 |
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