应用组织芯片研究凋亡抑制基因Survivin在胃癌中的表达

目的　检测Survivin和CD44v6在胃癌中的表达,以探讨二者在胃癌中表达的相关性及其与预后的关系。方法　利用组织芯片技术构建胃癌组织芯片,应用免疫组织化学S P法检测Survivin和CD44v6蛋白在胃癌中的表达。结果　胃癌中Survivin及CD44v6的总表达率分别为 58 6% (58 /99)和 35 4% (35 /99)。Survivin在早期胃癌中的表达显著高于进展期胃癌 (P<0 05 ),与胃癌的其它临床病理特征无相关性。CD44v6阳性表达与胃癌生长方式(P<0 001)、浸润深度 (P<0 05 )及淋巴结转移 (P<0 001 )密切相关。Sur vivin和CD44v6在胃癌中的表达无相关性(r=-0 065,P>0 05)。Survivin与胃癌患者的生存率无显著相关,而CD44v6表达阳性组 5年生存率(16 13% )显著低于CD44v6表达阴性组 5年生存率(60 67% ) (P<0 001 )。结论　Survivin和CD44v6在胃癌中均有过量表达。Survivin可能参与胃癌发生、发展的早期过程;而CD44v6阳性表达与胃癌的浸润和转移密切相关,同时可以提示胃癌的不良预后。

Study of the Expression of Survivin,an Anti-apoptosis Gene,in Human Gastric Cancers with Tissue Microarrays

Objective To investigate the prevalence and their clinical significance of survivin and CD44v6 in gastric carcinoma.Methods The gastric cancer tissue microarrays containing 93 gastric cancer specimen were used to determine survivin and CD44v6 expression by immunohistochemistry.Results The expression rates of survivin and CD44v6 were 58.6% (58/99) and 35.4% (35/99),respectively.The expression of survivin in advanced gastric carcinoma was significantly lower than it in early gastric carcinoma (P<0.05).No significant correlation between survivin expression and 5-years survival rate of patients with gastric carcinoma was observed.CD44v6 expression was found to be statistically associated with growth pattern (P<0.001),the depth of invasion (P<0.05) and lymph node metastasis (P<0.001).The 5-years survival rate was significantly lower in patients with CD44v6 positive tumors (16.13%) than in those with CD44v6 negative tumors (60.67%) (P<0.001).Conclusion Re-expression of survivin and CD44v6 is observed in gastric cancers.Survivin plays crucial role in early stage of human gastric carcinogenesis.The expression of CD44v6 may contribute to the invasion and the lymph node metastasis in gastric carcinoma and is a factor of poor clinical prognosis in patients with gastric carcinoma.