塞来昔布对人恶性淋巴瘤Raji细胞株增殖、凋亡及Survivin表达的影响

【目的】探讨塞来昔布对人恶性淋巴瘤Raji细胞的作用及其可能机制。【方法】体外培养人Burkitt’s淋巴瘤Raji细胞，用不同浓度塞来昔布进行干预。采用MTT比色法检测Raji细胞生长情况；流式细胞术分析塞来昔布对Raji细胞周期分布的影响并检测细胞凋亡及Survivin蛋白在细胞中的表达情况；应用RT-PCR法检测Raji细胞中Survivin mRNA的表达水平。【结果】塞来昔布对Raji细胞生长具有抑制作用，且在12．5～150μmol／L范围内呈时间、剂量依赖性；流式细胞术检测出典型的“凋亡峰”，凋亡率（9．20±0．19）％～（44．63±1．34）％；塞来昔布使Raji细胞G0／G1期细胞数增多，S和G2／M期细胞数减少，凋亡率和细胞周期分布呈量效依赖关系；塞来昔布下调Raji细胞中Survivin蛋白和Survivin mRNA的表达。【结论】塞来昔布抑制Raji细胞增殖、诱导其凋亡可能与阻滞细胞周期和下调Survivin表达有关。

Effects of Celecoxib on the proliferation,apoptosis and expression of Survivin in human lymphoma cell line Raji

Objective] To investigate the effect of Celecoxib（a selective COX-2 inhibitor）on Raji cell line of human malignant lymphoma and the possible mechanism. Methods] Human Burkitt＇ s lymphoma cells Raji were cultivated in vitro and treated with various concentrations of Celecoxib. The growth of Raji cells was determined by MTT assay. Apoptosis rate, cell cycle distribution and expression of Survivin protein in Raji cells were detected by FCM. Expression of Survivin mRNA in Raji cells was detected by RT-PCR. Results] The growth of Raji cells was inhibited by Celecoxib with concentration and time dependent manners from 12.5 - 150 μmol/L. Apoptosis peak was detected by FCM, and the apoptoticrate was between（9.20 ± 0.19）% and（44.63 ± 1.34）%. The cell percentage in G0/GI phase increased, whereas the cell percentage in S and G2/M phase decreased, the apoptotic rate and cell cycle distribution were in concentration dependent manners. Celecoxib downregulated the expression of Survivin protein and Survivin mRNA in Raji cells. Conclusions] The effect of Celecoxib inhibiting cell Raji and inducing cell apoptosis may be related to the blocking cell cycle progress and downregulating the expression of Survivin.