高效液相色谱法测定龙连利胆合剂中2组分的含量

目的:建立以高效液相色谱法测定龙连利胆合剂中大黄素、龙胆苦苷含量的方法。方法:色谱柱为ShimpackC18(150mm×6mm,5μm)。测定大黄素的流动相为甲醇-水-磷酸(850∶150∶0.3),检测波长为287nm;测定龙胆苦苷的流动相为甲醇-水(3∶7),检测波长为258nm。流速均为1.0mL.min-1。结果:大黄素的平均回收率为102.8%(RSD=1.7%,n=9),线性范围为0.05~0.25μg;龙胆苦苷的平均回收率为96.4%(RSD=1.7%,n=9),线性范围为0.125~1.0μg。结论:本方法准确、重现性好,可用于龙连利胆合剂的质量控制。

Determination of Emodin and Gentiopicrin in Longlianlidan Mixture by HPLC

OBJECTIVE: To establish an HPLC method for determination of Emodin and Gentiopicrin in Longlianlidan Mixture. METHODS: Different distilling methods were adopted to prepare the provided solvent for determination. The chromatographic column was Shimpack C18( 150mm× 6mm, 5μ m) . The mobile phase for determining Emodin consisted of methanol-water-phoshoric acid ( 850∶ 150∶ 0. 3) at a detection wavelength of 287nm, and that for determining Gentiopicrin consisted of methanol-water ( 3∶ 7) at a detection wavelength of 258nm, both with a flow rate of 1. 0mL· min-1. RESULTS: The average recovery of Emodin was 102. 8% ( RSD=1. 7% , n=9) with a linear range of 0. 05～ 0. 25μ g; That of Gentiopicrin was 96. 4% ( RSD=1. 7% , n=9) with a linear rang of 0. 125～ 1. 0μ g . CONCLUSION: The HPLC method is precise, reproducible and suitable for the quality control of Longlianlidan Mixture.