| 5-脱氧杂氮胞苷抑制肝癌细胞DNA 甲基转移酶转录 |
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| 引用本文： | 肖文华,刘丽华. 5-脱氧杂氮胞苷抑制肝癌细胞DNA 甲基转移酶转录[J]. 肿瘤防治研究, 2001, 28(4): 294-295 |
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| 作者姓名： | 肖文华 刘丽华 |
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| 作者单位： | 1. 第三军医大学附属西南医院肿瘤科;现在解放军304医院消化科
2. 第三军医大学附属西南医院肿瘤科, |
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| 摘 要： | 目的 明确 5- Aza- cd R对 DNA MTase转录水平的抑制作用。方法 用 5× 1 0 -7M的5- AZa- cd R处理肝癌细胞株 SMMC- 772 1和 H2和 Hep G2 ,就用 RT- PCR技术检测经药物处理前后 2株肝癌细胞 DNA MTase m RNA表达水平。结果 经 5- Aza- cd R处理后 ,2株肝癌细胞 DNAMTase m RNA表达量显著下降。结论 5- Aza- cd R能抑制肝细胞 DNA MTase转录 ,是其抗肿瘤作用的重要机制之一.
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| 关 键 词： | 肝癌细胞 5-脱氧杂氮胞苷 DNA 甲基转移酶 |
| 文章编号： | 1000-8578(2001)04-0294-02 |
| 收稿时间： | 2000-05-30 |
| 修稿时间： | 2000-05-30 |
Inhibition of DNA MTase at Transcription by 5-Aza-cdR in Hepatocellular CarcinomaCell line |
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| XIAO Wen hua,LIU Li hua Deptment of Oncology,Southwest Hospital,Third Military Medical University,Chongqing ,China. Inhibition of DNA MTase at Transcription by 5-Aza-cdR in Hepatocellular CarcinomaCell line[J]. Cancer Research on Prevention and Treatment, 2001, 28(4): 294-295 |
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| Authors: | XIAO Wen hua LIU Li hua Deptment of Oncology Southwest Hospital Third Military Medical University Chongqing China |
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| Affiliation: | XIAO Wen hua,LIU Li hua Deptment of Oncology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | Objective To under stant mechanism of inbhbition of DNA MTase by 5 Aza cdR.Methods detected the mRNA expression of DNA MTase gene by RT PCR in the hepatocellular carcinoma cell line SMMC 7721 and HepG 2 before and after treatment with 5 Aza cdR. Results In comparison with control group,mRNA expression are dramatically decreased at both of cell lines.Conclusion We concladed that 5 Aza cdR can inhibit the mRNA expression of DNA MTase. |
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| Keywords: | Hepatocellular Carcinoma cell 5 Aza 2' deoxycytine DNA methytransferase |
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