Apricot latent virus: a new species in the genus Foveavirus |
| |
Authors: | L G Nemchinov A M Shamloul E Z Zemtchik T D Verderevskaya A Hadidi |
| |
Institution: | (1) Vaccine and Therapeutic Development Section, Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, U.S.A., US;(2) Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, U.S.A., US;(3) Research Institute of Horticulture, Chisinau, Moldova, MD |
| |
Abstract: | Summary. Extraction of viral double-stranded RNA from peach leaves infected with Apricot latent virus (ALV) followed by molecular cloning of synthesized cDNA and its sequencing, suggested that ALV is a new virus, whose coat
protein (CP) coding region contains Apple stem pitting virus (ASPV)-related sequences. The sequenced portion of the ALV genome (1444 nt) includes the putative CP gene and the 3′ non-translated
region. The 5′ portion of this fragment (1-651 nt) is highly distinct whereas the 3′ portion is 77% identical to the corresponding
region of ASPV. Molecular hybridization experiments using a cRNA probe to ASPV with ALV-infected leaf tissue extracts also
revealed that the genome of ALV contains nucleotide sequences related to that of ASPV. Western blots of tissue extracts indicated
that ALV coat protein reacted with polyclonal antiserum against ASPV; however, the ALV CP differs in size from that of ASPV.
ALV was graft-transmitted to several Prunus rootstocks. Based on the available sequence data, serological observations and bioassays we propose that ALV is a new species
in the genus Foveavirus, typified by ASPV. ALV-specific PCR-primers and viral-specific cRNA probes developed in this investigation may be useful
for detecting the virus and for studying its epidemiology and geographical distribution.
Received February 10, 2000/Accepted April 3, 2000 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|