慢性氟中毒对大鼠精子活动度的影响

目的 探讨慢性氟中毒对大鼠精子活动度的影响,为氟的生殖毒性研究提供实验依据.方法 健康雄性Wistar大鼠32只,体质量150～180 g,按体质量随机分为4组:生理盐水(对照)组、低、中、高氟组(100、200、300mg·kg~(-1)·d~(-1)NaF),灌胃染毒90 d,每天称体质量.染毒结束后处死大鼠,摘取肝脏、肾脏、睾丸,称质量并计算脏器系数;取附睾游离精子,WLJY-9000型伟力彩色精子质量检测系统测定精子运动参数.结果 染毒第30天低、中氟组大鼠体质量(235.00±14.56)、(235.44±24.99)g]高于高氟组(206.00 ±18.16)g,P均<0.05)];第60、90天组大鼠体质量比较差异无统计学意义(F值分别为0.578、1.893,P均>0.05).大鼠肝脏系数、肾脏系数、睾丸系数组间比较差异无统计学意义(F值分别为2.148、0.907、1.801,P均>0.05).精子运动参数,平均路径速度(VAP)高氟组(25.04 ±4.59)μm/s]较对照组(20.22 ±3.29)μm/s]升高;直线速度(VSL)低、中、高氟组(18.82 ±3.19)、(17.84 ±4.54)、(16.46 ±2.63)μm/s]较对照组(12.48 ±1.73)μm/s]升高;直线性(LIN)低、中、高氟组(23.84±1.58)%、(24.99±3.37)%、(26.75 ±5.07)%]较对照组(33.29±4.00)%]降低,摆动性(WOB)中、高氟组(47.03 ±3.98)%、(49.21±7.73)%]较对照组(38.09 ±0.48)%]升高;平均移动角度(MAD)低氟组(68.29 ±5.71)度/s]较对照组(81.57 ±8.44)度/s]降低;鞭打频率(BCF)高氟组(11.47 ±0.61)次/s]较对照组(9.49 ±0.34)次/s]升高;精子密度(p)低、中氟组(1.26 ±0.24)×10~9、(1.84 ±0.50)×10~9/L]较对照组(3.94±1.10)×10~9个/L]降低(P均<0.05);曲线速度(VCL)、前向性(STR)、侧摆幅度(ALH),组间比较差异无统计学意义(F值分别为0.264、2.209、1.667,P均>0.05).结论 慢性氟中毒可影响大鼠精子活动度.降低精子密度.损害大鼠生殖系统.

Impact of fluorine poisoning on sperm motility of male rats

Objective To probe into the impact on sperm motility in male rat induced by fluorine poisoning, and provide experimental basis to further research for reproductive toxicity of fluoride. Methods According to bodyweight, 32 male Wistar rats were randomly divided into control group, the low-dose, medium-dose and high-dose group( 100,200,300 mg·kg~(-1)·d~(-1) NaF), and were treated by intragastric administration for 90 days, and the weight of the rats was observed each day. After the last intragastric administration, all rats were killed. The relative weight of liver, kidney and testis was calculated. Rat epididymides were plucked off and spermatozoa released from it. Sperm motility parameters were measured by WLJY-9000 color-detection system of sperm quality. Results Compared with high-dose group(206.00 ± 18.16)g], the weight of low-dose and medium-dose group (235.00 ± 14.56), (235.44 ± 24.99)g] in 30 days were statistically significant increased(all P < 0.05) ; there were no significant differences between the groups in 60 days and in 90 days(F = 0.578,1.893, all P > 0.05). Comparison of organ coefficient of liver, kidney and testis among three groups showed no significant difference(F = 2.148,0.907, 1.801, all P > 0.05). The average path velocity(VAP) of the high-dose group (25.04 ± 4.59)μm/s] showed significant increase compared with control group (20.22 ± 3.29)μm/s] ; the straight line velocity(VSL) of the low- dose, medium-dose and high-dose group (18.82± 3.19), (17.84 ± 4.54), (16.46 ± 2.63)μm/s] showed significant increase compared with control group ( 12.48 ± 1.73 ) μm/s ] ; linearity (LIN) of the low-dese, medium-dose and high.dose group(23.84±1.58)%,(24.99±3.37)%,(26.75±5.07)%]showed significant decrease compared with control group(33.29±4.00)%];wobble(WOB)of the medium-dose and high-dose group(47.03±3.98)%,(4921±723)%]showed significant increase compared with control group(38.09±0.48)%];mean angular deviation (MAD)of the low-dose group(68.29±5.71)radian/s]showed significant decrease compared with control group (81.57±8.44)radian/s];beat cross frequency(BCF)ofthe high-dose group(117±0.61)/s]showed significant increase compared with control group(9.49±0.34)/s];sperm density(p)of the low-dose and medium-dose group (1.26±0.24)×10~9/L,(1.84±0.50)×10~9/L]showed significant decrease compared with control group (3.94±1.10)×10~9/L,all P<0.05].Comparison of the eurvilinearvelocity(VCL),straightness(STR),amplitude of lateral head displacement(ALH)among three groups showed no significant difference(F=0.264,2.209,1.667, all P>0.05).Conclusion Fluorine poisoning could change sperm motility parameters of the rat,reduce the sperm density and cau8e damage to the reproductive system.