糖尿病与吡格列酮对心肌缺血再灌注损伤的影响及其机制

目的:观察大鼠心肌缺血再灌注(MIR)时超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-PX)、丙二醛(MDA)、Na ,K -ATP酶、Mg2 -ATP酶和Ca2 -ATP酶的变化及糖尿病与吡格列酮对其的影响,并探讨其可能机制.方法:将实验动物随机分为两组:对照组,进一步分为MIR组(C1组)和吡格列酮 MIR组(C2组);糖尿病组,也分为MIR组(D1组)和吡格列酮 MIR组(D2组).检测SOD,MDA,GSH-PX,Na ,K -ATP酶,Mg2 -ATP酶,Ca2 -ATP酶水平.结果:①与C1组相比,D1组血清和心肌的SOD、GSH-PX明显降低,MDA明显升高,心肌线粒体Na ,K -ATP酶活性明显降低;②与C1组相比,C2组血清和心肌的SOD,GSH-PX明显升高,MDA明显降低,心肌线粒体Na ,K -ATP酶、Mg2 -ATP酶、Ca2 -ATP酶活性明显升高;③与D1组相比,D2组血清和心肌的SOD,GSH-PX明显升高,MDA明显降低,心肌线粒体Na ,K -ATP酶、Mg2 -ATP酶、Ca2 -ATP酶活性明显升高.结论:糖尿病可加重MIR损伤,吡格列酮既可减轻MIR造成的损伤,也可减轻糖尿病MIR造成的损伤.

Investigate the effects and the reasons of diabetic and pioglitazone in myocardial ischemic-reperfusion injure

Objective: To observe the changes of superoxide dismutase,glutathione peroxidase,malonialdehyde,Na ,K -ATPase,Mg2 -ATPase and Ca2 -ATPase in myocardial ischemic reperfusion,and the effects of diabetic and pioglitazone in them;and to investigate the possible reason.Methods: Rats were randomly divided into two groups.One was the control group,which was subdivided into MIR group(C1 group)and pioglitazone MIR group(C2 group).Another was the diabetic group,which was subdivided into MIR group(D1 group)and pioglitazone MIR group(D2 group).The changes of MDA,SOD,GSH-PX,Na ,K -ATPase,Mg2 -ATPase and Ca2 -ATPase were examined. Results: Compared with C1 group,the levels of SOD,GSH-PX were decreased significantly,the levels of MDA were increased significantly,and the activities of Na ,K -ATPase in myocardial cytochrome were decreased significantly in D1 group;compared with C1 group,the levels of SOD,GSH-PX were increased significantly,the levels of MDA were decreased significantly,the activities of Na ,K -ATPase,Mg2 -ATPase,Ca2 -ATPase in myocardial cytochrome were increased significantly in C2 group;compared with D1 group,the levels of SOD,GSH-PX were increased significantly,the levels of MDA were decreased significantly,and the activities of Na ,K -ATPase,Mg2 -ATPase,Ca2 -ATPase in myocardial cytochrome were increased significantly in D2 group. Conclusion: The findings indicated that diabetic will aggravate the injury of MIR,pioglitazone will lighten the injury of MIR and MIR of diabetic rats.