膀胱癌端粒酶活性表达及其意义探讨

目的探讨膀胱癌的端粒酶活性表达及其在膀胱癌中的诊断价值。方法采用端粒重复序列扩增程序──酶标法（定量）和银染法（定性）对２８例膀胱瘤、１１例癌旁膀胱、６例正常膀胱的组织标本进行端粒酶活性检测。结果膀胱癌组织的端粒酶活性阳性检出率为８９．３％（２５／２８），癌旁膀胱组织为２７．３％（３／１１），正常膀胱组织为０％（０／６）。膀胱癌组与癌旁组、正常组端粒酶阳性检出率经比较，有显著性差异（Ｐ＜０．０１）。端粒酶活性表达与膀胱癌病理分期、分级等无相关性（Ｐ＞０．０５）。结论端粒酶激活发生在肿瘤形成的早期阶段，有望成为检测早期初发或复发膀胱癌有用的肿瘤标志物，癌旁组织端粒酶的激活提示肿瘤微浸润可能。

Telomerase activity in bladder carcinoma and its significance

Objective To investigate telomerase activity in bladder carcinoma and its diagnostic significance. MethodsTelomerase activity was examined by TRAP-ELISA (Telomeric repeat amplification protocol-ELISA,Quantitative) and TRAP-SilverStaining Assay (Qualitative) in tissue sample from 28 bladder carcinoma,11adjacent noncancerous bladders,6 normal bladders.Results The positive rate of telomerase activity was 89.3 % (25/28),27.3 % (3/11) and 0% (0/6) in bladder carcinoma, in adjacent non-cancerous bladder and in normal bladder respectively.The positive rate of telomerase activity in bladder carcinoma was signifi-cantly higher than that of adjacent noncancerous bladder and normal bladder (P<0.01).No correlation was found between telom-erase activity and the staging or grading etc of the tumors (P>O.05).Conclusions The activation of telomerase occurs in the earlystages of carcinogenesis and could perhaps be a useful tumor marker for the detection of early primal or recurrent bladdercarcinoma. The activation of telomerase in the paracancer tissue shows the possibility of tumor microinvasion.