Caspase-9和Bax在胃癌中的表达以及与凋亡的关系

目的：探讨Caspase-9和Bax在胃癌发展中的作用以及二者的表达与细胞凋亡的关系。方法：应用免疫组织化学S-P法检测Caspase-9和Bax在57例胃癌及48例非癌胃黏膜组织的表达,用原位末端标记法（TUNEL法）检测相应胃组织细胞凋亡。结果：Caspase-9蛋白在胃癌组织中阳性表达率为40.35%,较非癌胃黏膜组织的阳性表达率70.83%明显降低（P〈0.05）;Bax蛋白在胃癌组织中阳性表达率为35.09%,明显低于非癌胃黏膜组织的72.92%（P〈0.05）。Caspase-9蛋白阳性表达者细胞凋亡指数（AI）值（11.20±3.39）%与其阴性者AI值（6.22±2.45）%比较差异有显著性（P〈0.05）;Bax蛋白阳性表达者AI值（12.54±3.60）%,与其阴性者AI值（7.18±1.25）%比较差异有显著性（P〈0.05）。在不同类型胃癌组织中,高中分化腺癌、低分化腺癌、黏液癌的AI值分别为（5.72±1.25）%、（2.54±0.98）%、（3.28±1.42）%,其中高中分化腺癌和后二者之间有统计学差异（P〈0.05）。结论：Caspase-9和Bax可能与胃癌的发生或发展相关;推测Bax激活Caspase-9的线粒体通道受抑可能参与胃癌的发生。

Expression of Caspase-9 and Bax in Stomach Cancer and Its Relation to Cell Apoptosis

Objective： To explore the effects of caspase-9 and bax in the development ot stomach cancer and the relation of their expression to cell apoptosis. Methods： Expression of caspase-9 and bax in 57 specimens of stomach cancer and 48 specimens of non-cancer stomach tissue was detected with immunohistochemistry S-P method, and the cell apoptosis in stomach tissue detected with TUNEL method. Results： The positive expression rate of caspase-9 in stomach canser tissue （40.35%） was significantly lower （P 〈 0.05） than that in non-cancer stomach tissue （70.83%）. The positive expression rate of bas in stomach canser tissue （35.09%） was significantly lower （P 〈 0.05） than that in non-cancer stomach tissue （72.92%）. The apoptosis index （AI） of easpase-9 positive eases （ 11.20 ± 3.39 ） % was different from that of caspase-9 negative cases （6.22 ± 2.45 ） %, and the difference was significant （P 〈 0.05 ）. The AI of bax positive cases （12.54± 3.60）% was different from that of bax negative cases （7.18 ± 1.25 ） %, and the difference was significant （P 〈 0.05 ）. AI values in well-differentiated adenocarcinoma （WDA）, poorly differentiated adenocarcinoma （PDA）, and colloid carcinoma （ CC ） were （ 5.72±1.25 ） %, （2.54 ± 0.98 ） %, and （ 3.28 ± 1.42） % respectively. There were significant differences between WDA AI and PDA AI, and between WDA AI and CC AI （P 〈0. 05）. Conclusions： Caspase-9 and bax might be related to the occurrence and development of stomach cancer. The inhibition of mitochondrial channel for caspase-9 stimuted by bax might participate the initiation of stomach cancer.