BH3-only蛋白在紫杉醇诱导胃腺癌细胞凋亡中的表达

目的研究BH3-only蛋白Bim、Nora、Puma在紫杉醇诱导胃腺癌细胞凋亡中的表达水平及其意义。方法流式细胞仪Annexin Ⅴ／PI双染法检测细胞凋亡率；半定量RT-PCR和Western blot分别检测在紫杉醇诱导前后Bim、Noxa、Puma的mRNA和蛋白的表达水平。结果紫杉醇诱导胃腺癌细胞凋亡具有剂量和时间依赖性，SGC-7901胃腺癌细胞比BGC-823细胞对紫杉醇更具敏感性。Bim的mRNA和蛋白的表达水平在紫杉醇诱导的SGC-7901中有显著升高，而在BGC-823中无明显变化。Noxa的mRNA表达水平在紫杉醇诱导的SGC-7901及BGC-823中有一过性升高，而其蛋白表达水平却检测不到。两种胃腺癌细胞系均未检测到Puma mRNA的表达。结论紫杉醇能通过诱导细胞凋亡杀伤胃腺癌细胞，而此凋亡可能与BH3-only蛋白Bim、Noxa的表达上调有关，而与Puma mRNA的表达无关。

BH3-only protein expression in apoptosis of paclitaxel-induced gastric adenocarcinoma cells and its significance

Objective To study the expression level and significance of BH3-only protein Bim, Noxa, Puma in apoptosis of paclitaxel-induced gastric adenocarcinoma cells. Methods Apoptosis was measured with flow cytometry using Annexin V and propidium iodide staining. The expression of Bim, Noxa and Puma at mRNA and protein levels before and after paclitaxel treatment were measured using semi-quantitative PCR and Western blot analysis, respectively. Results Paclitaxel induced apoptosis of gastric adenocarcinoma cells in a dose- and time-dependent manner. It appeared that SGC-7901 cells were more sensitive than BGC-823 cells to paclitaxel-induced apoptosis. The expression of Bim at both mRNA and protein levels was markedly increased in SGC-7901 cells after exposure to paclitaxel. In contrast, there was no significant change in expression of Bim in BGC-823 cells before and after treatment. The expression of Noxa at both mRNA and protein levels was temporarily increased in both cell lines after exposure to paclitaxel. The expression of Puma mRNA was not observed in both cell lines. Conclusion Paclitaxel kills gastric adenocarcinoma cells by inducing Of apoptosis, which may be associated with up-regulation of Bim and Noxa but not Puma.