Suppressive effect of ethanol on the expression of hepatic asialoglycoprotein receptors augmented by interleukin-1β, interleukin-6, and tumor necrosis factor-α |
| |
Authors: | Junji Kato Yoshihiro Mogi Yutaka Kohgo Rishu Takimoto Masayoshi Kobune Hiroyuki Hisai Tokiko Nakamura Kohichi Takada Yoshiro Niitsu |
| |
Affiliation: | (1) Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan, JP;(2) Naganuma Municipal Hospital, Naganuma, Japan, JP;(3) Third Department of Internal Medicine, Asahikawa Medical College, Asahikawa, Japan, JP |
| |
Abstract: | Blood levels of inflammatory-related cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, are elevated in patients with alcoholic liver diseases. We investigated the effects of these cytokines and ethanol on the expression of hepatic asialoglycoprotein receptors (AGPRs) in a human hepatoblastoma cell line, HepG2. An [125I]-asialo-orosomucoid binding assay showed significant increases in surface AGPR numbers in HepG2 cells by treatment with IL-1β, IL-6, and TNF-α, to levels which were approximately 130% of the values in untreated control cells. However, the enhanced AGPR numbers induced by treatment with these cytokines were markedly suppressed, to 70%–80% of the number in the untreated cells, by treatment with ethanol. Immunological detection of AGPR with a specific antibody demonstrated that the modulation of surface AGPR numbers was correlated with the cellular expression levels of AGPR. These results suggest that, although IL-1β, IL-6, and TNF-α stimulate the synthesis of hepatic AGPR, ethanol suppresses the expression of AGPR augmented by these cytokines. This leads to an increase in serum asialo-orosomucoid levels caused by the disordered catabolism mediated by AGPR in patients with alcoholic liver disease. (Received Dec. 5, 1997; accepted May 22, 1998) |
| |
Keywords: | : ethanol asialoglycoprotein receptor IL-1β IL-6 TNF-α |
本文献已被 SpringerLink 等数据库收录! |
|