| 吡那地尔预处理对缺氧/复氧成后年大鼠心肌细胞内钙离子浓度的影响 |
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| 引用本文： | 邓胜利,姚刚,张琳,喻田. 吡那地尔预处理对缺氧/复氧成后年大鼠心肌细胞内钙离子浓度的影响[J]. 贵州医药, 2010, 34(12). DOI: 10.3969/j.ISSN.1000-744X.2010.12.001 |
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| 作者姓名： | 邓胜利 姚刚 张琳 喻田 |
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| 作者单位： | 贵州省遵义医学院,麻醉学系,563003 |
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| 基金项目： | 贵州省教育厅自然科学研究培育项目(编号:黔教科(2007)038号)遵义医学院硕±启动基金(编号:院字 |
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| 摘 要： | 目的观察吡那地尔(Pinacidil)预处理对缺氧/复氧后成年大鼠心肌细胞内Ca2+浓度([Ca2+]i)的影响,以探讨吡那地尔在缺氧/复氧中抑制钙超载作用的机制。方法通过Langen-dorff离体心脏灌注装置,用Ⅱ型胶原酶对心脏逆行灌注,进行成年大鼠心肌细胞的分离与培养,建立心肌细胞缺氧/复氧模型。实验分组及处理:(1)正常组(NOR组):细胞正常培养;(2)缺氧/复氧组(CON组):细胞在95%N2+5%CO2培养箱中缺氧45 min,复氧1 h;(3)吡那地尔组(Pina组):先加入吡那地尔(30μmol/L)预处理15 min,再缺氧45 min,复氧1h;(4)格列苯脲拮抗Pina组(Gi+Pina组):先用格列苯脲(50μmol/L)处理5 min后,余处理同Pina组。通过Fluo-3/AM荧光指示剂负载,用激光共聚焦显微镜检测心肌细胞Ca2+浓度变化。结果 NOR组心肌细胞内钙离子荧光强度值较低;细胞缺氧45 min,复氧1 h后其荧光强度值与NOR组相比显著增高(P<0.01);而Pina组细胞内荧光强度值较CON组及Gi+Pina组低(P<0.01)。结论心肌细胞缺氧/复氧后...
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| 关 键 词： | 吡那地尔 缺氧/复氧 心肌细胞 钙 |
Effect of Pinacidil preconditioning OR calcium concentration of cultured adult rat cardiomyocytes during hypoxia/reoxygenation |
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| Deng Shengli,Yao Gang,Zhang Lin,Yu Tian. Effect of Pinacidil preconditioning OR calcium concentration of cultured adult rat cardiomyocytes during hypoxia/reoxygenation[J]. Guizhou Medical Journal, 2010, 34(12). DOI: 10.3969/j.ISSN.1000-744X.2010.12.001 |
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| Authors: | Deng Shengli Yao Gang Zhang Lin Yu Tian |
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| Affiliation: | Deng Shengli,Yao Gang,Zhang Lin,Yu Tian.Department of Anesthesiology,Zunyi Medical College,Zunyi 563003,China |
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| Abstract: | Objective To investigate the effect of Pinacidil preconditioning on calcium concentration of cultured adult rat cardiomyocytes after hypoxia/reoxygenation,and to search for the mechanism of repressed intracellular calcium concentration by Pinacidil.Methods Adult rat cardiomyocytes were separated and cultured by Langendorff isolated heart perfusion apparatus.The cultured cells were randomly divided into 4 groups which were normal group(NOR),control group(CON),Pinacidil preconditioning group(Pina) and Glibenc... |
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| Keywords: | Pinacidil Hypoxia/reoxygenation Cardiac muscle cell Calcium |
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