亚砷酸体外对人肝癌细胞株BEL-7402影响的初步研究

目的 体外培养人肝癌细胞株BEL－7402，从多个角度探讨三氧化二砷（As2O3）的抗肿瘤作用及其机制。方法 应用倒置相差显微镜、电子显微镜、透谢电镜、流式细胞仪，分别对不同浓度加药组及对照组BEL－7402细胞的存活。形态学改变，细胞DNA含量的分布进行了观察和测定。结果 0.5、1、2μmol/L As2O3均能抑制人肝癌细胞株BEL－7402细胞的生长增殖。流式细胞仪分析显示，加药组在G1期细胞前均出现亚二倍体峰，且G0／G1期细胞减少，S期细胞增多；电镜下，对照组细胞核质比大、核大、核膜有明显切迹，0.5μmol/L As2O3组细胞核质比减少、核变圆、胞浆内出现分化良好的细胞器， 0.5、1、2μmol/L As2O3组均可见细胞膜完整、核固缩、凋亡小体形成。结论 三氧化二砷不仅抑制人肝癌细胞增殖，而且诱导细胞凋亡。

Effect of arsenic trioxide to human hepatoma cell line BEL-7402 cultured in vitro

Objective To study the effect of a wide range concentration ofarsenic trioxide on human hepatoma cell line BEL-7402 and its mechanism using the method of human hepatoma cell line BEL-7402 cultured in vitro.Methods The BEL-7402 cells were treated with arsenic trioxide(final concentration 0.5,1,2μmol/L,respectively) on variable duration or 4 successive days.The cell growth and proliferation were observed by cell counting and cell-growth curve.Morphologic changes were studied with electron microscopy.Flow cytometry was used to assay cell-DNA distribution.Results The cell growth was significantly inhibited by the different concentration of arsenic trioxide as revealed by cell counting and cell-growth curve.Arsenic trioxide treatment at 0.5,1,2μmol/L resulted in a sub-G1 cell peak.The decrease of G0/G1 phase cell and the increased percentage of S phase cell were observed by flow cytometer,which suggested that the inhibition effect of arsenic trioxide on BEL-7402 cell laid in G0/G1 phase cell.Apoptotisrelated morphology,such as intace cell membrane,nucleic condensation,apoptotic body formation,could be seen under the electron microscopy more significant.Conclusion The arsenic trioxide not only inhibites proliferation but also induces apoptosis of human hepatoma cell line BEL-7402.