甲型H1N1病毒HA、NA氨基酸序列比较及抗原性表位预测 |
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引用本文: | 邹泽红,张俊艳,刘兆宇,刘昌文,李胜涛,何颖.甲型H1N1病毒HA、NA氨基酸序列比较及抗原性表位预测[J].现代保健,2013(19):1-3. |
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作者姓名: | 邹泽红 张俊艳 刘兆宇 刘昌文 李胜涛 何颖 |
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作者单位: | 广州医学院第二附属医院呼吸疾病国家重点实验室变态反应研究室广州市过敏反应与临床免疫重点实验室,广东广州510260 |
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基金项目: | 国家自然基金(编号:30771240) |
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摘 要: | 目的:比较新型甲型H1N1流感病毒分离株HA、NA氨基酸序列之间的差异,分析HA、NA蛋白可能的抗原性细胞表位。方法:从GenBank中选取28株世界各地的新型甲型H1N1流感病毒分离株并下载各株HA、NA的氨基酸序列;使用ClustalX和MEGA2.0软件对氨基酸序列的进行进化树分析;用Protean软件预测HA、NA蛋白的B细胞表位;用NetMHCⅡ2.2预测T细胞抗原表位。结果:世界范围的毒株之间氨基酸序列相对保守,HA及NA均只有少数位点的变异,HA蛋白氨基酸序列之间较NA蛋白氨基酸序列之间变异较大;预测HA蛋白具有8个B细胞表位和10个T细胞表位;NA蛋白有12个B细胞表位和7个T细胞表位。结论:HA、NA蛋白的少数区域抗原性相对比较稳定,可以作为检测以及疫苗研究的靶区域。
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关 键 词: | H1N1流感病毒 抗原表位 血凝素 神经氨酸酶 |
Comparison of HA,NA Amino Acid Sequences of Novel Influenza Virus A/H1N1 and Prediction of Antigenic Epitopes |
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Institution: | ZOU Ze-hong,ZHANG Jun-yan,LIU Zhao-yu et al Guangzhou Municipal Key Laboratory of Allergy & Clinical Immunology,Allergy Research Branch of the State Key Laboratory of Respiratory Disease,The Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260,China |
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Abstract: | Objective:To compare the amino acid sequences difference of HA,NA novel influenza virus A/H1N1 isolates,and decipher possible B cell epitopes and T cell epitopes of HA,NA protein.Method:The amino acid sequences of 28 novel influenza A/H1N1 virus isolates around the world were downloaded from Genbank. Phylogenetic trees were constructed based on the amino acid sequences of HA and NA by using software Clustal X and MEGA 2.0.B cell and T cell epitopes were respectively predicted with Protean software and NetMHC Ⅱ 2.2 online server.Result:HA protein sequences showed a relatively larger variation comparing to NA protein sequences,nevertheless both of them each had very less divergent amino acid residues. Software predictions revealed the presence of 8 B cell epitopes and 10 T cell epitopes randomly distributed throughout HA sequence and 12 B cell epitopes and 7 T cell epitopes throughout NA sequence. Conclusion:The antigenicity of HA,NA protein is relatively stable in certain regions which can be used as the target area in diagnosis and vaccine research. |
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Keywords: | Influenza virus A/H1N1 Antigenic epitope Hemagglutinin Neuraminidase |
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