儿童急性淋巴细胞白血病抗原错译表达的临床意义

目的 研究儿童急性淋巴细胞白血病(ALL)抗原错译表达的临床意义。方法 对54例ALL患儿的骨髓标本分别进行细胞形态学及细胞化学染色，确定其FAB类型，运用一组相关的单克隆抗体，采用流式细胞仪及直接免疫荧光标记技术进行免疫分型，采用吉姆萨G显带技术进行核型分析。结果 儿童ALL54例髓系抗原阳性表达率为29.63％，其中CD13为25．93％，CD33为20．37％，CD14为11．11％。T—ALL和B—ALL髓系抗原阳性表达差异无统计学意义(30．77％vs529．27％ P＝0.918)。CD34表达阳性ALL髓系抗原阳性表达率明显高于CD34表达阴性ALL髓系抗原阳性表达率(40.63％vs13.64％P＝0．039)；ALL髓系抗原阳性表达与髓系抗原阴性表达的完全缓解(CR)率差异无统计学意义(81．25％vs94．74％ P＝0．148)；但生存率曲线比较分析，ALL髓系抗原阳性表达的生存时间短(P＝0.031)。结论 儿童ALL的抗原错译表达率为29．63％；CD34阳性的ALL抗原错译表达率明显高于CD34表达阴性的ALL抗原错译表达率；ALL髓系抗原阳性表达的生存时间短；ALL髓系相关抗原阳性表达可能是影响ALL患儿预后的不利因素。

Aberration antigen expression of acute lymphoblastic leukemia in children

Objective To study the aberration antigen expression of childhood acute lymphoblastic leukemia(ALL)and its clinical implication.Methods Bone marrow specimens of 54 ALL patients were evaluated to prove the diagnosis and classification by morphologic,cytochemical,immunologic and cytogenetic examinations.Results The incidence of myeloid antigen expression in 54 childhood ALL was 29.63 %.Among them,CD 13 was 25.93 %,CD 33 was 20.37 % and CD 14 was 11.11 %,no difference was found in expression of myeloid antigen between B-ALL and T-ALL(30.77 % vs 29.2 % P=0.918).Myeloid antigen were expressed munch higher in CD 34 positive ALL than CD 34 negative ALL(40.63 % vs 13.6 % P=0.039).Though complete remission rate was no difference between myeloid antigen positive ALL and myeloid antigen negative ALL(81.25 % vs 94.74 % P= 0.148),ALL children with myeloid antigen expression had shorter survival than those without myeloid antigen expression(P=0.031).Conclusions Aberration antigen expression in childhood ALL is about 29.63 %.Aberration antigen is much higher in CD 34 positive ALL than CD 34 negative ALL.ALL children with aberration antigen have shorter survival than those without aberration antigen.Aberration antigen may influence prognostic of childhood ALL.