电脉冲裸基因肌肉注射胰岛素样生长因子-1介导的小鼠肺纤维化病理观察

目的：观察胰岛素样生长因子-1(IGF-1)所致小鼠肺纤维化的病理变化。方法：应用电泳冲裸基因肌肉注射法，观察注射截短型IGF1后2，3，4及8周组小鼠肺组织病理学变化。结果：2周组小鼠肺间质充血水肿。3周组肺泡隔明显增厚，大量肺泡上皮细胞，巨噬细胞及成纤维细胞增生。4周组肺间质大量胶原沉积，肺纤维化样改变。8周组肺纤维化病变明显减轻，肺泡结构渐趋恢复。结论：IGF-1可直接导致肺纤维化发生。IGF-1介导的肺纤维化早期病变可以逆转。

Observation on pulmonary fibrosis in mice after electric pulse-mediated insulin-like growth factor-1 gene transfer into skeletal muscle

Objective To observe the pathological changes induced by Insulin like growth factor 1 (IGF 1) by electric pulse in the development of murine pulmonary fibrosis. Methods Truncated IGF 1 [pcDI Des(1-3)IGF 1] was injected into the skeletal muscles of the mice by electric pulses through invasive needle electrodes. Lung tissues were obtained at the end of week 2, 3, 4, and 8 respectively after injection. The pathological changes in the lungs were observed with light and electron microscopy. Results Edema and congestion were noted in the lung interstitium at the end of week 2. The interalveolar septums were significantly thickened with proliferations of pneumocytes, macrophages and fibroblasts at the end of week 3. Remarked collagen deposition in the interstitium and significant interstitial fibrosis were observed at the end of week 4. At the end of week 8, the interstitial fibrosis subsided and the structure of alveolar wall returned to normal. Conclusion IGF 1 causes directly pulmonary fibrosis in mice, but the early lung injury is reversible.